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      Aptamer-Based Nanoporous Anodic Alumina Interferometric Biosensor for Real-Time Thrombin Detection

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          Abstract

          Aptamer biosensors are one of the most powerful techniques in biosensing. Achieving the best platform to use in aptamer biosensors typically includes crucial chemical modifications that enable aptamer immobilization on the surface in the most efficient manner. These chemical modifications must be well defined. In this work we propose nanoporous anodic alumina (NAA) chemically modified with streptavidin as a platform for aptamer immobilization. The immobilization of biotinylated thrombin binding aptamer (TBA) was monitored in real time by means of reflective interferometric spectroscopy (RIfS). The study has permitted to characterize in real time the path to immobilize TBA on the inner pore walls of NAA. Furthermore, this study provides an accurate label-free method to detect thrombin in real-time with high affinity and specificity.

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          Aptamers as targeted therapeutics: current potential and challenges

          Jiehua Zhou, John M. Rossi (2016)
          Nucleic acid aptamers offer several advantages over traditional antibodies, but their clinical translation has been delayed by several factors, including insufficient potency, lack of safety data and high production costs. Here, Zhou and Rossi provide an overview of aptamer generation, focusing on recent technological advances and clinical development, as well as challenges and lessons learned.
          Article 0 comments Cited 482 times – based on 0 reviews     Review now
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            Introduction to biosensors

            Nikhil Bhalla, Pawan Jolly, Nello Formisano (2016)
            Biosensors are nowadays ubiquitous in biomedical diagnosis as well as a wide range of other areas such as point-of-care monitoring of treatment and disease progression, environmental monitoring, food control, drug discovery, forensics and biomedical research. A wide range of techniques can be used for the development of biosensors. Their coupling with high-affinity biomolecules allows the sensitive and selective detection of a range of analytes. We give a general introduction to biosensors and biosensing technologies, including a brief historical overview, introducing key developments in the field and illustrating the breadth of biomolecular sensing strategies and the expansion of nanotechnological approaches that are now available.
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              An overview of foodborne pathogen detection: in the perspective of biosensors.

              Vijayalakshmi Velusamy, Khalil Arshak, Olga Korostynska … (2010)
              Food safety is a global health goal and the foodborne diseases take a major crisis on health. Therefore, detection of microbial pathogens in food is the solution to the prevention and recognition of problems related to health and safety. For this reason, a comprehensive literature survey has been carried out aiming to give an overview in the field of foodborne pathogen detection. Conventional and standard bacterial detection methods such as culture and colony counting methods, immunology-based methods and polymerase chain reaction based methods, may take up to several hours or even a few days to yield an answer. Obviously this is inadequate, and recently many researchers are focusing towards the progress of rapid methods. Although new technologies like biosensors show potential approaches, further research and development is essential before biosensors become a real and reliable choice. New bio-molecular techniques for food pathogen detection are being developed to improve the biosensor characteristics such as sensitivity and selectivity, also which is rapid, reliable, effective and suitable for in situ analysis. This paper not only offers an overview in the area of microbial pathogen detection but it also describes the conventional methods, analytical techniques and recent developments in food pathogen detection, identification and quantification, with an emphasis on biosensors. Copyright 2009 Elsevier Inc. All rights reserved.
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                Author and article information

                Journal
                Journal ID (nlm-ta): Sensors (Basel)
                Journal ID (iso-abbrev): Sensors (Basel)
                Journal ID (publisher-id): sensors
                Title: Sensors (Basel, Switzerland)
                Publisher: MDPI
                ISSN (Electronic): 1424-8220
                Publication date (Electronic): 19 October 2019
                Publication date Collection: October 2019
                Volume: 19
                Issue: 20
                Electronic Location Identifier: 4543
                Affiliations
                Departament d’Enginyeria Electrònica, Elèctrica i Automàtica, ETSE, Universitat Rovira i Virgili, Avda. Països Catalans 26, 43007 Tarragona, Spain; laura.pol@ 123456urv.cat (L.P.); laurakaren.acosta@ 123456urv.cat (L.K.A.); josep.ferre@ 123456urv.cat (J.F.-B.)
                Author notes
                [* ]Correspondence: lluis.marsal@ 123456urv.cat ; Tel.: +34-977-55-96-25
                Author information
                https://orcid.org/0000-0001-5653-4552
                https://orcid.org/0000-0002-5210-5133
                https://orcid.org/0000-0002-5976-1408
                Article
                Publisher ID: sensors-19-04543
                DOI: 10.3390/s19204543
                PMC ID: 6833485
                PubMed ID: 31635027
                SO-VID: 7cee0f3d-d159-4829-b602-ac2a8614d217
                Copyright © © 2019 by the authors.
                License:

                Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license ( http://creativecommons.org/licenses/by/4.0/).

                History
                Date received : 13 September 2019
                Date accepted : 15 October 2019
                Categories
                Subject: Article

                ScienceOpen disciplines: Biomedical engineering
                Keywords: nanoporous anodic alumina,streptavidin,aptamers,thrombin,rifs,biosensing
                Data availability:
                ScienceOpen disciplines: Biomedical engineering
                Keywords: nanoporous anodic alumina, streptavidin, aptamers, thrombin, rifs, biosensing

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