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      The New Clubroot Resistance Locus Is Located on Chromosome A05 in Chinese Cabbage (Brassica rapa L.)

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          Is Open Access

          Primer3—new capabilities and interfaces

          Polymerase chain reaction (PCR) is a basic molecular biology technique with a multiplicity of uses, including deoxyribonucleic acid cloning and sequencing, functional analysis of genes, diagnosis of diseases, genotyping and discovery of genetic variants. Reliable primer design is crucial for successful PCR, and for over a decade, the open-source Primer3 software has been widely used for primer design, often in high-throughput genomics applications. It has also been incorporated into numerous publicly available software packages and web services. During this period, we have greatly expanded Primer3’s functionality. In this article, we describe Primer3’s current capabilities, emphasizing recent improvements. The most notable enhancements incorporate more accurate thermodynamic models in the primer design process, both to improve melting temperature prediction and to reduce the likelihood that primers will form hairpins or dimers. Additional enhancements include more precise control of primer placement—a change motivated partly by opportunities to use whole-genome sequences to improve primer specificity. We also added features to increase ease of use, including the ability to save and re-use parameter settings and the ability to require that individual primers not be used in more than one primer pair. We have made the core code more modular and provided cleaner programming interfaces to further ease integration with other software. These improvements position Primer3 for continued use with genome-scale data in the decade ahead.
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            Rapid isolation of high molecular weight plant DNA

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              Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations.

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                Author and article information

                Journal
                Russian Journal of Genetics
                Russ J Genet
                Pleiades Publishing Ltd
                1022-7954
                1608-3369
                March 2018
                March 28 2018
                March 2018
                : 54
                : 3
                : 296-304
                Article
                10.1134/S1022795418030080
                7d708d4c-911d-47e2-8b7e-d10b899d9db7
                © 2018

                http://www.springer.com/tdm

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