Ectopic ACTH Syndrome: Discrepancy between Somatostatin Receptor Status in vivo and ex vivo, and between Immunostaining and Gene Transcription for POMC and CRH

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Abstract

Objective: To characterize somatostatin receptor status in vivo and ex vivo and immunoreactivity and gene transcription for proopiomelanocortin (POMC) and corticotropin-releasing hormone (CRH) in a case of Cushing’s syndrome caused by a sporadic metastatic medullary thyroid carcinoma (MTC). Methods:<sup>111</sup>In octreoscan, analysis of tumorous mRNA transcripts for somatostatin receptor subtypes (SSTR) as well as for POMC and CRH. Results: The tissue was intensely positive by <sup>111</sup>In octreoscan but expressed only SSTR 1, 3 and 5. There was immunopositivity only for CRH, but gene transcription for both POMC and CRH was seen. Conclusions: (1) This first comparison between somatostatin receptor status in vivo and ex vivo in MTC shows a marked positive octreoscan despite absent SSTR 2 expression, and (2) this is the first report of a discrepancy between immunostaining and gene transcription for POMC and CRH.

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The Diagnosis and Differential Diagnosis of Cushing's Syndrome and Pseudo-Cushing's States

J Newell-Price (1998)

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Gene transcription of receptors for growth hormone-releasing peptide and somatostatin in human pituitary adenomas.

S. Nielsen, S Mellemkjaer, L Rasmussen … (1998)

Growth hormone (GH)-releasing peptides (GHRP) or secretagogs (GHS) constitute a family of synthetic compounds with potent and specific GH releasing activity. The receptor (GHS-R) has recently been cloned even though the endogenous ligand remains to be identified. GHRPs act both at the hypothalamic and the pituitary level through mechanisms involving amplification of GH-releasing hormone activity and functional somatostatin antagonism. In the present study we examined the co-expression of messenger RNA (mRNA) for GHS-R and all 5 somatostatin receptor subtypes (sstr 1-5) in 28 human pituitary tumors by RT-PCR. GHS-R transcription was detected in 11 out of 12 somatotroph adenomas and in 2 out of 2 prolactinomas, whereas GHS-R expression was detected in only 2 out of 14 clinically nonfunctioning adenomas (NFPA), and no expression was seen in the only ACTH secreting adenoma. Almost all tumors expressed sstr 2 mRNA (n = 24), whereas only 1 tumor expressed sstr 4 mRNA. The expression of sstr 3 mRNA was inversely associated with GHS-R expression (P < 0.001), which could be attributed to a high prevalence of sstr 3 expression in NFPA. This study suggests that GHS-R expression is predominantly observed in somatotroph adenomas and much less so in NFPA. Moreover, the presence of a distinct pattern of somatostatin receptor subtype co-expression is suggested, which may provide a molecular basis for the complex interaction between GHRPs and somatostatin.

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Journal ID (publisher-id): HRE

Journal ID (nlm-ta): Horm Res Paediatr

Journal ID (doi): 10.1159/issn.1663-2818

Title: Hormone Research in Paediatrics

Publisher: S. Karger AG

ISSN (Print): 1663-2818

ISSN (Electronic): 1663-2826

Publication date Subscription-year: 2002

Publication date (Print): 2002

Publication date (Electronic): 30 May 2002

Volume: 57

Issue: 5-6

Pages: 200-204

Affiliations

^aDepartment of Endocrinology and Metabolism, ^bLaboratory for Molecular Pathology, ^cDepartment of Nuclear Medicine, and ^dMedical Department M (Endocrinology and Diabetes), Aarhus University Hospital, Aarhus, Denmark; ^eDepartment of Pathology, University of Toronto, Ont., Canada

Article

Publisher ID: 58383 Other ID: Horm Res 2002;57:200–204

DOI: 10.1159/000058383

PubMed ID: 12053094

SO-VID: 7d71b029-d627-49a0-9911-fe4c18bd84a5

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Ectopic ACTH Syndrome: Discrepancy between Somatostatin Receptor Status in vivo and ex vivo, and between Immunostaining and Gene Transcription for POMC and CRH

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Most cited references 2

The Diagnosis and Differential Diagnosis of Cushing's Syndrome and Pseudo-Cushing's States

Gene transcription of receptors for growth hormone-releasing peptide and somatostatin in human pituitary adenomas.

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