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      Influence of NH 4CI on Polarized Release of Endogenous Protein Degradation Products and on Morphology in LLC-PK 1 Cells

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          Abstract

          Increased renal ammoniagenesis is thought to be a causative factor for renal hypertrophy which occurs in several disorders accompanied by metabolic acidosis. We studied the influence of ammonia on the polarized release of degradation products of endogenous proteins in LLC-PK<sub>1</sub> cells. Release of acid-soluble radioactivity to the extracellular space decreased under the influence of NH<sub>4</sub>Cl with a remarkable transient reduction of basolateral release. Electron microscopically NH<sub>4</sub>Cl-treated cells showed numerous enlarged lysosomes suggesting an accumulation of incompletely degraded cytoplasmatic material in the lysosomal compartment. We conclude that split products of lysosomal degradation of endogenous proteins are preferentially transported to the extracellular space via the basolateral plasma membrane.

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          Author and article information

          Journal
          AJN
          Am J Nephrol
          10.1159/issn.0250-8095
          American Journal of Nephrology
          S. Karger AG
          0250-8095
          1421-9670
          2000
          February 2000
          13 January 2000
          : 20
          : 1
          : 74-81
          Affiliations
          aDepartment of Pathology, University of Bonn, bDivision of Nephrology, Department of Internal Medicine, University of Würzburg, and cDivision of Clinical Chemistry, Department of Gynecology and Obstetrics, University of Bonn, Germany
          Article
          13561 Am J Nephrol 2000;20:74–81
          10.1159/000013561
          10644874
          © 2000 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          Page count
          Figures: 7, Tables: 3, References: 25, Pages: 8
          Product
          Self URI (application/pdf): https://www.karger.com/Article/Pdf/13561
          Categories
          Laboratory Investigations

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