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      Influence of NH 4CI on Polarized Release of Endogenous Protein Degradation Products and on Morphology in LLC-PK 1 Cells

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          Increased renal ammoniagenesis is thought to be a causative factor for renal hypertrophy which occurs in several disorders accompanied by metabolic acidosis. We studied the influence of ammonia on the polarized release of degradation products of endogenous proteins in LLC-PK<sub>1</sub> cells. Release of acid-soluble radioactivity to the extracellular space decreased under the influence of NH<sub>4</sub>Cl with a remarkable transient reduction of basolateral release. Electron microscopically NH<sub>4</sub>Cl-treated cells showed numerous enlarged lysosomes suggesting an accumulation of incompletely degraded cytoplasmatic material in the lysosomal compartment. We conclude that split products of lysosomal degradation of endogenous proteins are preferentially transported to the extracellular space via the basolateral plasma membrane.

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          Author and article information

          Am J Nephrol
          American Journal of Nephrology
          S. Karger AG
          February 2000
          13 January 2000
          : 20
          : 1
          : 74-81
          aDepartment of Pathology, University of Bonn, bDivision of Nephrology, Department of Internal Medicine, University of Würzburg, and cDivision of Clinical Chemistry, Department of Gynecology and Obstetrics, University of Bonn, Germany
          13561 Am J Nephrol 2000;20:74–81
          © 2000 S. Karger AG, Basel

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          Page count
          Figures: 7, Tables: 3, References: 25, Pages: 8
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          Laboratory Investigations


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