5
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: not found

      Visualisation of Microglia with the use of Immunohistochemical Double Staining Method for CD-68 and Iba-1 of Cerebral Tissue Samples in Cases of Brain Contusions.

      Read this article at

      ScienceOpenPublisherPubMed
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          In the recent years it has been confirmed that the main component of the immune response in an injury of the nerve cell comes from microglia and macrophages. The main challenge in the field of microglia research is to detect the different stages of cellular activation by visualization of the cell morphology. The existing visualization techniques are based on surface molecules expression in resting and activated microglia cells. For visualization of the microglial cells and their functional state we used double labeling method for cd-68 and iba1 in brain contusions with different survival time. Microglia are stained brown with Iba-1, whereas microglia impregnated with black, grainy color, represents activated microglia stained with CD 68. We had significantly positive results, and we were able to observe changes in the morphology of the microglia that correlated with the survival time. Using double labeling with Iba-1 and cd68 we were able to determine their physiological state based on the morphology and immunoreactivity.

          Related collections

          Author and article information

          Journal
          Pril (Makedon Akad Nauk Umet Odd Med Nauki)
          Prilozi (Makedonska akademija na naukite i umetnostite. Oddelenie za medicinski nauki)
          Walter de Gruyter GmbH
          1857-9345
          1857-9345
          2015
          : 36
          : 2
          Article
          /j/prilozi.2015.36.issue-2/prilozi-2015-0062/prilozi-2015-0062.xml
          10.1515/prilozi-2015-0062
          27442380
          7d98c410-d5fe-4cba-9ed8-ac727058e206
          History

          Comments

          Comment on this article