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The perichromatin region of the plant cell nucleus is the area with the strongest co-localisation of snRNA and SR proteins

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      Abstract

      The spatial organisation of the splicing system in plant cells containing either reticular ( Allium cepa) or chromocentric ( Lupinus luteus) nuclei was studied by immunolabelling of SR proteins, snRNA, and the PANA antigen, known markers for interchromatin granule clusters in mammalian cells. Electron microscope results allowed us to determine the distribution of these molecules within the structural domains of the nucleus. Similar to animal cells, in both plant species SR proteins were localised in interchromatin granules, but contrary to animal cells contained very small amounts of snRNA. The area with the strongest snRNA and SR protein co-localisation was the perichromatin region, which may be the location of pre-mRNA splicing in the plant cell nuclei. The only observable differences in the organisation of reticular and chromocentric nuclei were the size of the speckles and the number of snRNA pools in the condensed chromatin. We conclude that, despite remarkable changes in the nuclear architecture, the organisation of the splicing system is remarkably similar in both types of plant cell nuclei.

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      The online version of this article (doi:10.1007/s00425-012-1640-z) contains supplementary material, which is available to authorized users.

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      Most cited references 44

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          Genomes are more than linear sequences. In vivo they exist as elaborate physical structures, and their functional properties are strongly determined by their cellular organization. I discuss here the functional relevance of spatial and temporal genome organization at three hierarchical levels: the organization of nuclear processes, the higher-order organization of the chromatin fiber, and the spatial arrangement of genomes within the cell nucleus. Recent insights into the cell biology of genomes have overturned long-held dogmas and have led to new models for many essential cellular processes, including gene expression and genome stability.
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            Author and article information

            Affiliations
            [1 ]Department of Cell Biology, Institute of General and Molecular Biology, Nicolaus Copernicus University, Ul. Gagarina 9, 87-100 Torun, Poland
            [2 ]Department of Plant Physiology and Biotechnology, Nicolaus Copernicus University, 87-100 Torun, Poland
            [3 ]Department of Pathology, USC Keck School of Medicine, Los Angeles, CA USA
            [4 ]Division of Inflammation Biology, La Jolla Institute for Allergy and Immunology, La Jolla, CA 92037 USA
            Contributors
            +48-56-6114454 , +48-56-6114772 , janiaszn@umk.pl
            Journal
            Planta
            Planta
            Planta
            Springer-Verlag (Berlin/Heidelberg )
            0032-0935
            1432-2048
            24 April 2012
            24 April 2012
            August 2012
            : 236
            : 2
            : 715-726
            3404291
            22526497
            1640
            10.1007/s00425-012-1640-z
            © The Author(s) 2012
            Categories
            Original Article
            Custom metadata
            © Springer-Verlag 2012

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