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      Open Design 3D-Printable Adjustable Micropipette that Meets the ISO Standard for Accuracy

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      , , *
      Micromachines
      MDPI
      open source labware, 3D printing, functional prototyping

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          Abstract

          Scientific communities are drawn to the open source model as an increasingly utilitarian method to produce and share work. Initially used as a means to develop freely-available software, open source projects have been applied to hardware including scientific tools. Increasing convenience of 3D printing has fueled the proliferation of open labware projects aiming to develop and share designs for scientific tools that can be produced in-house as inexpensive alternatives to commercial products. We present our design of a micropipette that is assembled from 3D-printable parts and some hardware that works by actuating a disposable syringe to a user-adjustable limit. Graduations on the syringe are used to accurately adjust the set point to the desired volume. Our open design printed micropipette is assessed in comparison with a commercial pipette and meets the ISO 8655 standards.

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          Most cited references40

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          Materials science. Building research equipment with free, open-source hardware.

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            Configurable 3D-Printed millifluidic and microfluidic 'lab on a chip' reactionware devices.

            We utilise 3D design and 3D printing techniques to fabricate a number of miniaturised fluidic 'reactionware' devices for chemical syntheses in just a few hours, using inexpensive materials producing reliable and robust reactors. Both two and three inlet reactors could be assembled, as well as one-inlet devices with reactant 'silos' allowing the introduction of reactants during the fabrication process of the device. To demonstrate the utility and versatility of these devices organic (reductive amination and alkylation reactions), inorganic (large polyoxometalate synthesis) and materials (gold nanoparticle synthesis) processes were efficiently carried out in the printed devices.
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              Foldscope: Origami-Based Paper Microscope

              Here we describe an ultra-low-cost origami-based approach for large-scale manufacturing of microscopes, specifically demonstrating brightfield, darkfield, and fluorescence microscopes. Merging principles of optical design with origami enables high-volume fabrication of microscopes from 2D media. Flexure mechanisms created via folding enable a flat compact design. Structural loops in folded paper provide kinematic constraints as a means for passive self-alignment. This light, rugged instrument can survive harsh field conditions while providing a diversity of imaging capabilities, thus serving wide-ranging applications for cost-effective, portable microscopes in science and education.
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                Author and article information

                Journal
                Micromachines (Basel)
                Micromachines (Basel)
                micromachines
                Micromachines
                MDPI
                2072-666X
                18 April 2018
                April 2018
                : 9
                : 4
                Affiliations
                Department of Bioengineering, University of Illinois at Chicago, Chicago, IL 60607, USA; mbrenn3@ 123456uic.edu (M.D.B.); fbokha2@ 123456uic.edu (F.F.B.)
                Author notes
                [* ]Correspondence: dte@ 123456uic.edu ; Tel.: +1-312-355-3278
                Article
                micromachines-09-00191
                10.3390/mi9040191
                6187506
                7e16b470-36c7-4f2f-86a9-794b00fd5dc3
                © 2018 by the authors.

                Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license ( http://creativecommons.org/licenses/by/4.0/).

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                open source labware,3d printing,functional prototyping

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