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      Transcriptome analyses of the Giardia lamblia life cycle

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          Abstract

          We quantified mRNA abundance from 10 stages in the Giardia lamblia life cycle in vitro using Serial Analysis of Gene Expression (SAGE). 163 abundant transcripts were expressed constitutively. 71 transcripts were upregulated specifically during excystation and 42 during encystation. Nonetheless, the transcriptomes of cysts and trophozoites showed major differences. SAGE detected co-expressed clusters of 284 transcripts differentially expressed in cysts and excyzoites and 287 transcripts in vegetative trophozoites and encysting cells. All clusters included known genes and pathways as well as proteins unique to Giardia or diplomonads. SAGE analysis of the Giardia life cycle identified a number of kinases, phosphatases, and DNA replication proteins involved in excystation and encystation, which could be important for examining the roles of cell signaling in giardial differentiation. Overall, these data pave the way for directed gene discovery and a better understanding of the biology of G. lamblia. Copyright 2010 Elsevier B.V. All rights reserved.

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          Author and article information

          Journal
          Molecular and Biochemical Parasitology
          Molecular and Biochemical Parasitology
          Elsevier BV
          01666851
          November 2010
          November 2010
          : 174
          : 1
          : 62-65
          Article
          10.1016/j.molbiopara.2010.05.010
          2972195
          20570699
          7e220932-ccc0-4064-a7f1-3ad9ea577de2
          © 2010

          https://www.elsevier.com/tdm/userlicense/1.0/

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