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      Involvement of Immunopathogenic Mechanisms in a Spontaneously Occurring Glomerulopathy in Mice

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          Mice have been found to be susceptible to spontaneous renal localization of immune deposits. However, the significance of these immune deposits is still debated. We investigated the immunopathogenesis of a naturally occurring glomerulopathy associated with progressive proteinuria and glomerulosclerosis in 75 BALB/c mice. The mice were divided into five groups of 15 and killed at the age of 1, 3, 6, 12, or 18 months for laboratory and renal pathologic studies. These mice showed persistently increasing serum levels of immune complexes, a marked increase of glomerular immune deposits which were capable of fixing C3, and interstitial infiltration of lymphocytes and plasma cells, followed by the occurrence of proteinuria, mesangiopathy, and glomerulosclerosis. Our findings suggest that an immune system mediated process occurred in the kidneys of the mice tested.

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          A fluorometric assay for the measurement of nitrite in biological samples.

          The increasing importance of nitric oxide synthase has been underscored by the elucidation of its role in a growing number of normal and pathophysiological processes. Therefore, techniques for detection of nitrite/nitrate, oxidation products of the enzymatic conversion of arginine to citrulline and nitric oxide, should serve as useful tools in defining the contribution of NO synthase to these processes. We have developed a rapid and sensitive fluorometric assay for quantification of nitrite/nitrate based upon the reaction of nitrite with 2,3-diaminonaphthalene to form the fluorescent product, 1-(H)-naphthotriazole. The assay can be used to detect 10 nM nitrite, making it 50-100 times more sensitive than the well-known Griess assay. Moreover, the assay is adaptable to a 96-well plate format, facilitating the handling of a large number of samples including conditioned media from cell culture or the nitrite generated by the purified enzyme. Nitrite/nitrate levels in blood can also be monitored using this assay when it is combined with a filtration step (to remove hemoglobin) followed by conversion of the nitrate to nitrite by nitrate reductase. Thus, this fluorometric method combines speed and sensitivity with the handling of a large number of samples for the quantification of nitrite generated from in vivo and in vitro sources.
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            The case for intrarenal hypertension in the initiation and progression of diabetic and other glomerulopathies.

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              Age-associated changes in renal glomeruli of mice


                Author and article information

                S. Karger AG
                January 1998
                19 December 1997
                : 78
                : 1
                : 63-72
                a Division of Experimental Pathology, Department of Pathology and b Division of Nephrology, Department of Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei, c Graduate Institute of Life Sciences and d Department of Biochemistry, National Defense Medical Center, Taipei, e Department of Pathology, Chang-Gung Memorial Hospital, Taipei, Taiwan, ROC
                44884 Nephron 1998;78:63–72
                © 1998 S. Karger AG, Basel

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                Page count
                Figures: 12, References: 56, Pages: 10
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