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      Radioimmunoassay for Serum and Urinary Tamm-Horsfall Glycoprotein in the Rat

      ,

      Nephron

      S. Karger AG

      Tamm-Horsfall glycoprotein, Radioimmunoassay, Kidney, Serum, Urine, Homogenates

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          Abstract

          Rat Tamm-Horsfall glycoprotein (THG) was isolated from urine and disaggregated by gel filtration. Purity was confirmed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and autoradiography. Antibodies were raised and used in the development of a specific radioimmunoassay for rat THG. Parallelism could be demonstrated between the standard preparation and urine (diluted to 1:320 with distilled water) and serum (at all dilutions) samples. Recovery of standard added to urine or serum was on average 93% and inter- and intra-assay variation were both below 7%. There was a positive correlation between the mean urinary excretory rate of THG (μg/24h) and body weight in female rats (p < 0.001) but not in male rats. Serum THG levels were similar in both sexes and showed a positive correlation with body weight (p < 0.001; range 43–240 ng/ml). It is concluded that the rat urinary THG excretion rate is similar to that in man and that rat serum also contains a THG-like protein.

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          Author and article information

          Journal
          NEF
          Nephron
          10.1159/issn.1660-8151
          Nephron
          S. Karger AG
          1660-8151
          2235-3186
          1985
          1985
          04 December 2008
          : 40
          : 3
          : 362-367
          Affiliations
          Department of Paediatrics, Childrens Hospital, Sheffield, UK
          Article
          183494 Nephron 1985;40:362–367
          10.1159/000183494
          4010853
          © 1985 S. Karger AG, Basel

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          Page count
          Pages: 6
          Categories
          Original Paper

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