LncRNA NRON alleviates atrial fibrosis via promoting NFATc3 phosphorylation.

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Abstract

The aim of this study was to explore the role of NRON in the atrial fibrosis. The expression of NRON in atrial tissue was detected using qRT-PCR. The protein levels of collagen I, collagen III, NFATc3 and p-NFATc3 were determined by western blot. Immunohistochemistry assay were performed to observe expression and distribution of collagen I in atrial tissues. The atrial fibroblasts were authenticated by vimentin/troponin immunofluorescence staining. Fibroblast proliferation was detected by CCK-8 assay. The morphological changes of cardiac tissue were observed by HE staining. Myocardial fibrosis was detected by masson staining. NRON expression was significantly downregulated in atrial tissues of AF. NRON suppressed fibroblast proliferation; expression of collagen I and collagen III; activation of NFATc3 and nucleu import. NRON promoted p-NFATc3 expression and alleviated atrial fibrosis in vivo. Our data indicated that NRON alleviates atrial fibrosis via promoting NFATc3 phosphorylation.

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Author and article information

Journal

Journal ID (iso-abbrev): Mol. Cell. Biochem.

Title: Molecular and cellular biochemistry

Publisher: Springer Science and Business Media LLC

ISSN (Electronic): 1573-4919

ISSN (Print): 0300-8177

Publication date (Electronic): Jul 2019

Volume: 457

Issue: 1-2

Affiliations

[1 ] Department of Cardiac Intensive Care Unit, CICU, The First Affliated Hospital of Anhui Medical University, Hefei, 230022, Anhui, People's Republic of China.

[2 ] Thoracic Surgery, Cardiacmacrovascular Surgery, Shaoxing people's Hospital, Shaoxing, 312000, Zhejiang, People's Republic of China.

[3 ] Department of Cardiovascular Surgery, The First Affliated Hospital of Anhui Medical University, 218 Jixi Road, Hefei, 230022, Anhui, People's Republic of China.

[4 ] Department of Cardiovascular Surgery, The First Affliated Hospital of Anhui Medical University, 218 Jixi Road, Hefei, 230022, Anhui, People's Republic of China. shenglin_ge@sohu.com.

[5 ] Department of Cardiovascular Surgery, The First Affliated Hospital of Anhui Medical University, 218 Jixi Road, Hefei, 230022, Anhui, People's Republic of China. guo_zhixiang@sina.com.

Article

Publisher Item ID: 10.1007/s11010-019-03521-y

DOI: 10.1007/s11010-019-03521-y

PubMed ID: 30895498

SO-VID: 7e8adeb9-3b6f-4634-ab12-ccdb75c4132d

History

Keywords: NRON,NFATc3 phosphorylation,Atrial fibrosis

Data availability:

Keywords: NRON, NFATc3 phosphorylation, Atrial fibrosis

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