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      BLISS: A Bioorthogonal Dual-Labeling Strategy to Unravel Lignification Dynamics in Plants.

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          Abstract

          A better in vivo understanding of lignin formation within plant cell walls will contribute to improving the valorization of plant-derived biomass. Although bioorthogonal chemistry provides a promising platform to study the lignification process, methodologies that simultaneously detect multiple chemical reporters in living organisms are still scarce. Here, we have developed an original bioorthogonal labeling imaging sequential strategy (BLISS) to visualize and analyze the incorporation of both p-hydroxyphenyl (H) and guaiacyl (G) units into lignin in vivo with a combination of strain-promoted and copper-catalyzed azide-alkyne cycloadditions. On our path to BLISS, we designed a new azide-tagged monolignol reporter for H units in metabolic lignin engineering and used it in conjunction with an alkyne-tagged G unit surrogate to study lignification dynamics in flax. Here, we show that BLISS provides precise spatial information on the zones of active lignification and reveals polarization in single-cell lignification dynamics.

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          Author and article information

          Journal
          Cell Chem Biol
          Cell chemical biology
          Elsevier BV
          2451-9456
          Mar 16 2017
          : 24
          : 3
          Affiliations
          [1 ] Université de Lille, CNRS, UMR 8576 - UGSF - Unité de Glycobiologie Structurale et Fonctionnelle, 59000 Lille, France. Electronic address: cedric.lion@univ-lille1.fr.
          [2 ] Université de Lille, CNRS, UMR 8576 - UGSF - Unité de Glycobiologie Structurale et Fonctionnelle, 59000 Lille, France.
          [3 ] Université de Lille, Inserm, U1003 - PHYCEL - Physiologie Cellulaire, 59000 Lille, France.
          [4 ] Université de Lille, CNRS, UMR 8576 - UGSF - Unité de Glycobiologie Structurale et Fonctionnelle, 59000 Lille, France. Electronic address: simon.hawkins@univ-lille1.fr.
          Article
          S2451-9456(17)30039-9
          10.1016/j.chembiol.2017.02.009
          28262560
          7f4cbb47-a7d0-4215-96f8-8780fbeff97e
          History

          CuAAC,SPAAC,bioorthogonal chemistry,click chemistry,dual labeling,lignification,lignin,metabolic labeling,monolignol,plant cell wall

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