Draft Genome Sequence of a Hospital-Associated Clone of Klebsiella pneumoniae ST340/CC258 Coproducing RmtG and KPC-2 Isolated from a Pediatric Patient

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Abstract

We report here the draft genome sequence of a Klebsiella pneumoniae strain 1194/11, belonging to the hospital-associated sequence type 340 (ST340; clonal complex CC258), isolated from a catheter tip culture from a pediatric patient. The multidrug-resistant strain coproduced the 16S rRNA methyltransferase rRNA RmtG and β-lactamases KPC-2 and CTX-M-15.

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A5-miseq: an updated pipeline to assemble microbial genomes from Illumina MiSeq data.

David Coil, Guillaume Jospin, Aaron Darling (2015)

Open-source bacterial genome assembly remains inaccessible to many biologists because of its complexity. Few software solutions exist that are capable of automating all steps in the process of de novo genome assembly from Illumina data.

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The route of antimicrobial resistance from the hospital effluent to the environment: focus on the occurrence of KPC-producing Aeromonas spp. and Enterobacteriaceae in sewage.

Eloiza Campana, Ana Assis, Renata C Picão … (2013)

We investigated the antimicrobial resistance profile and the occurrence of Klebsiella pneumoniae carbapenemase (KPC)-producing Gram-negative rods in sewage samples obtained from a Brazilian teaching hospital and from the wastewater treatment plant (WWTP) that receives it for treatment. We identified multidrug-resistant bacteria as well as KPC-2-producing Aeromonas spp. and several Enterobacteriaceae species, including Kluyvera spp., in the hospital effluent and in different sites of the WWTP. Most isolates showed the blaKPC-2 gene harbored on a transposon that was carried by conjugative plasmids. The presence of KPC production among Aeromonas spp., Kluyvera spp., and other Enterobacteriaceae indicates the adaptability of such isolates to aquatic environments, not only in the hospital effluent but also throughout the WWTP. Although secondary treatment seems to decrease the amount of KPC producers in sewage, multidrug-resistant isolates are continually disposed in the urban river. Thus, sewage treatment regulations are urgently needed to decelerate the evolution of antimicrobial resistance beyond hospitals. Copyright © 2013 Elsevier Inc. All rights reserved.

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Update of the molecular epidemiology of KPC-2-producing Klebsiella pneumoniae in Brazil: spread of clonal complex 11 (ST11, ST437 and ST340).

Ana Carvalho-Assef, Gislaine Pereira, Marise Asensi … (2013)

To perform molecular epidemiology for 113 KPC-producing Klebsiella pneumoniae isolated in 2010 from 12 Brazilian states. The resistance profile was determined by disc diffusion and Etest. Genetic polymorphism was analysed by PFGE and multilocus sequence typing. The genetic environment of the bla(KPC) gene was determined by PCR and identification of the carrier plasmid was determined by hybridization. Most of the isolates were multidrug resistant, with 15% and 49% being resistant to polymyxin and tigecycline, respectively. Twenty-two sequence types (STs) were observed, with ST11, ST437 and ST340 [clonal complex 11 (CC11)] being the most prevalent (75% of isolates) observed in 10 states. bla(KPC-2) was associated with transposon Tn4401 'b' and in 36% this gene was found in IncN plasmids of 40 kb. In Brazil, the spread of bla(KPC-2) is occurring due to dispersion of Tn4401 'b', carried by IncN plasmids of 40 kb, and mainly the dissemination of CC11, with ST437 and ST11 playing an important role.

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Author and article information

Journal

Journal ID (nlm-ta): Genome Announc

Journal ID (iso-abbrev): Genome Announc

Journal ID (hwp): ga

Journal ID (pmc): ga

Journal ID (publisher-id): GA

Title: Genome Announcements

Publisher: American Society for Microbiology (1752 N St., N.W., Washington, DC )

ISSN (Electronic): 2169-8287

Publication date (Electronic): 3 November 2016

Publication date Collection: Nov-Dec 2016

Volume: 4

Issue: 6

Electronic Location Identifier: e01130-16

Affiliations

[a ]Department of Clinical Analysis, School of Pharmacy, Universidade de São Paulo, São Paulo, Brazil

[b ]Center of Bacteriology, Instituto Adolfo Lutz, São Paulo, Brazil

[c ]Genome Investigation and Analysis Laboratory (GENIAL), Institute of Biomedical Sciences, Universidade de São Paulo, São Paulo, Brazil

[d ]Department of Microbiology, Institute of Biomedical Sciences, Universidade de São Paulo, São Paulo, Brazil

Author notes

Address correspondence to Louise Cerdeira, lcerdeira@ 123456usp.br , or Nilton Lincopan, lincopan@ 123456usp.br .

Author information

Nilton Lincopan http://orcid.org/0000-0003-0161-5800

Article

Publisher ID: genomeA01130-16

DOI: 10.1128/genomeA.01130-16

PMC ID: 5095459

PubMed ID: 27811089

SO-VID: 7f6a8841-ac0e-42d2-b0a3-6f7c24ae7d95

License:

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

History

Date received : 18 August 2016

Date accepted : 12 September 2016

Page count

Figures: 0, Tables: 0, Equations: 0, References: 7, Pages: 2, Words: 1012

Funding

Funded by: MCTI | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) http://dx.doi.org/10.13039/501100003593

Award Recipient : Nilton Lincopan

Funded by: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) http://dx.doi.org/10.13039/501100002322

Award Recipient : Miriam Rodriguez Fernandes

Funded by: Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) http://dx.doi.org/10.13039/501100001807

Award Recipient : Louise Teixeira Cerdeira Award Recipient : Gabriela Rodrigues Francisco Award Recipient : Maria Fernanda Campagnari Bueno Award Recipient : Doroti de Oliveira Garcia

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cover-date November/December 2016

ScienceOpen disciplines: Genetics

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ScienceOpen disciplines: Genetics

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Draft Genome Sequence of a Hospital-Associated Clone of Klebsiella pneumoniae ST340/CC258 Coproducing RmtG and KPC-2 Isolated from a Pediatric Patient

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Most cited references 5

A5-miseq: an updated pipeline to assemble microbial genomes from Illumina MiSeq data.

The route of antimicrobial resistance from the hospital effluent to the environment: focus on the occurrence of KPC-producing Aeromonas spp. and Enterobacteriaceae in sewage.

Update of the molecular epidemiology of KPC-2-producing Klebsiella pneumoniae in Brazil: spread of clonal complex 11 (ST11, ST437 and ST340).

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