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      Plant voltage-dependent anion channels are involved in host defense against Pseudomonas cichorii and in Bax-induced cell death.

      Plant Cell Reports

      Amino Acid Sequence, Cell Death, physiology, DNA, Complementary, genetics, Gene Expression Regulation, Plant, Gene Silencing, Hydrogen Peroxide, analysis, Mitochondria, metabolism, Molecular Sequence Data, Plant Proteins, Pseudomonas, pathogenicity, RNA, Plant, Sequence Alignment, Tobacco, microbiology, Voltage-Dependent Anion Channels, bcl-2-Associated X Protein

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          The voltage-dependent anion channel (VDAC) is a major outer mitochondrial membrane protein. It is well documented that VDAC plays an important role in apoptosis, a kind of programmed cell death, in mammalian systems. However, little is known about the role of the plant counterpart during the process of plant-specific cell death such as pathogen-induced hypersensitive response. To address this issue, we isolated three VDAC full-length cDNAs (NtVDAC1-3) from Nicotiana tabacum. The deduced products, NtVDACs, share 78-85% identity and retain the conserved eukaryotic mitochondrial porin signature distal to their C-terminal regions. Mitochondrial localization of three NtVDACs in plant cells was confirmed via a green fluorescent protein fusion method. Then, we addressed the main issue concerning pathogenesis relation. The N. benthamiana orthologues of NtVDACs were upregulated by challenge with the non-host pathogen Pseudomonas cichorii, but not after challenge with the virulent pathogen P. syringae pv. tabaci. Both the pharmaceutical inhibition of VDAC and silencing of NbVDACs genes compromised the non-host resistance against P. cichorii, suggesting the involvement of VDACs in defense against non-host pathogen. Involvement of NbVDACs in Bax-mediated cell death was also verified using a similar approach.

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