1 July 2018
2-ODD, 2-oxolutarate-dependent dioxygenase, APX, ascorbate peroxidase, AO, ascorbate oxidase, DHA, dehydroascorbate (bicyclic), DHAR, dehydroascorbate reductase, GLUT, DHA transporter, GSH, glutathione, GSSG, glutathione disulfide, MDHA, monodehydroascorbate, MDHAR, monodehydroascorbate reductase, SOD, superoxide dismutase, SVCT, sodium-dependent ascorbate transporter, VDE, violaxanthin de-epoxidase, Ascorbic acid, Vitamin C, Hydrogen peroxide, Ascorbate peroxidase, Ascorbate oxidase, Gulonolactone oxidase, Galactonolactone dehydrogenase, Dehydroascorbate, Monodehydroascorbate, Dioxygenase, Iron reduction, Epigenetics, vtc mutants
Ascorbic acid is synthesised by eukaryotes, the known exceptions being primates and some other animal groups which have lost functional gulonolactone oxidase. Prokaryotes do not synthesise ascorbate and do not need an ascorbate supply, so the functions that are essential for mammals and plants are not required or are substituted by other compounds. The ability of ascorbate to donate electrons enables it to act as a free radical scavenger and to reduce higher oxidation states of iron to Fe 2+. These reactions are the basis of its biological activity along with the relative stability of the resulting resonance stabilised monodehydroascorbate radical. The importance of these properties is emphasised by the evolution of at least three biosynthetic pathways and production of an ascorbate analogue, erythroascorbate, by fungi. The iron reducing activity of ascorbate maintains the reactive centre Fe 2+ of 2-oxoglutarate-dependent dioxygenases (2-ODDs) thus preventing inactivation. These enzymes have diverse functions and, recently, the possibility that ascorbate status in mammals could influence 2-ODDs involved in histone and DNA demethylation thereby influencing stem cell differentiation and cancer has been uncovered. Ascorbate is involved in iron uptake and transport in plants and animals. While the above biochemical functions are shared between mammals and plants, ascorbate peroxidase (APX) is an enzyme family limited to plants and photosynthetic protists. It provides these organisms with increased capacity to remove H 2O 2 produced by photosynthetic electron transport and photorespiration. The Fe reducing activity of ascorbate enables hydroxyl radical production (pro-oxidant effect) and the reactivity of dehydroascorbate (DHA) and reaction of its degradation products with proteins (dehydroascorbylation and glycation) is potentially damaging. Ascorbate status influences gene expression in plants and mammals but at present there is little evidence that it acts as a specific signalling molecule. It most likely acts indirectly by influencing the redox state of thiols and 2-ODD activity. However, the possibility that dehydroascorbylation is a regulatory post-translational protein modification could be explored.
The function and metabolism of ascorbate is compared between plants and mammals.
Ascorbate readily reduces radicals and Fe/Cu.
Ascorbate is synthesised by plants, protists and animals via different pathways.
Fungi synthesise the analogue D-erythroascorbate.
Plants and photosynthetic protists use ascorbate peroxidase to remove H 2O 2.