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      Effect of Chloroquine on Thyroliberin Interaction with Clonal Rat Prolactin Cells

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          Abstract

          The lysosomotropic agent, chloroquine, has been used to investigate the implication of lysosomal activity and membrane traffic in TRH binding, TRH internalization and TRH-induced stimulation of prolactin secretion in a rat prolactin cell line (GH3/B6). Chloroquine by itself does not affect cell number, cell protein and basal prolactin secretion. It does not alter TRH binding and internalization as well as both effects of TRH on the stimulation of prolactin secretion, i.e., prolactin release and prolactin production. In contrast, chloroquine partially inhibits the spontaneous dissociation of (<sup>3</sup>H)TRH from cells previously loaded with (<sup>3</sup>H)TRH and reduces prolactin release following TRH withdrawal. In addition the kinetic pattern of the TRH dissociation is modified in a manner which suggests that TRH is bound to different intracellular compartments. Chloroquine, nevertheless, does not alter the TRH-induced down regulation of (<sup>3</sup>H)TRH binding sites. Electron microscopic observations and acid phosphatases localization reveal that chloroquine elicits a disorganization of the Golgi zone and accumulation of membrane whorls within large vacuoles. This suggests that the effects of chloroquine on TRH interaction with GH3 cells may be mediated by an inhibition of membrane recycling.

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          Author and article information

          Journal
          NEN
          Neuroendocrinology
          10.1159/issn.0028-3835
          Neuroendocrinology
          S. Karger AG
          0028-3835
          1423-0194
          1982
          1982
          26 March 2008
          : 34
          : 3
          : 180-190
          Affiliations
          Groupe de Neuroendocrinologie Cellulaire, Collège de France, Paris, France
          Article
          123298 Neuroendocrinology 1982;34:180–190
          10.1159/000123298
          6803181
          © 1982 S. Karger AG, Basel

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          Page count
          Pages: 11
          Categories
          Original Paper

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