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      Comparison of Net Fluxes of Li and Na in Vascular Smooth Muscle

      research-article
      Journal of Vascular Research
      S. Karger AG
      Vascular smooth muscle, Vascular cations, Blood vessels, Sodium

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          Abstract

          The rat tail artery enriched by incubation in a lithium-substituted medium loses Li at three distinct rates upon return to a normal medium at 37 °C. The first is very rapid with a single exponential rate constant of the order of 1 min<sup>–1</sup>, while the second and third are considerably slower with rate constants of the order of 3 h<sup>–1</sup> and 0.6 h<sup>–1</sup>, respectively. Both slow components are cellular since they combine into a single component when active Na transport is inhibited by the absence of external K, by ouabain, or by low temperature. None of these procedures affects the rapid loss of Li from extracellular locations. It follows that cell Li can be measured simply by washing out extracellular Li at 2 °C, a procedure which removes only a small definable amount from cells. The influx of Li into cells during incubation in Li-substituted medium follows 1st order kinetics and exchanges 1:1 with cell K after a delay of about 20 min at 37 °C. This delay in influx is associated with active Na transport since it is abolished by the absence of external K, by low temperature, and by ouabain. The activation energy required for the influx of Li and of Na in K-free media is consistent, in the main, with passive diffusion through a restricted area although there is a suggestion in the case of Na that an additional mechanism (e.g. carrier) may be involved.

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          Author and article information

          Journal
          JVR
          J Vasc Res
          10.1159/issn.1018-1172
          Journal of Vascular Research
          S. Karger AG
          1018-1172
          1423-0135
          1975
          1975
          18 September 2008
          : 12
          : 4
          : 219-235
          Affiliations
          Department of Anatomy, University of British Columbia, Vancouver
          Article
          158058 Blood Vessels 1975;12:219–235
          10.1159/000158058
          803b8c03-2b40-4d44-b0f2-333202d247c5
          © 1975 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          History
          : 25 November 1974
          : 07 March 1975
          Page count
          Pages: 17
          Categories
          Paper

          General medicine,Neurology,Cardiovascular Medicine,Internal medicine,Nephrology
          Vascular smooth muscle,Vascular cations,Blood vessels,Sodium

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