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      Gas chromatographic determination of phenol in fish tissues as a phenyl acetate derivative following solvent extraction of acidified samples

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          Abstract

          This study aimed to develop a chromatographic method to quantitatively determine phenol in fish tissues. This method involves solvent extraction of acidified samples, followed by derivatization to phenyl acetate and analysis with gas chromatography coupled with mass spectrometry (GC–MS). Phenol in a representative tissue sample (belly, gill, or renal tubules), which was homogenized with 2 N sulfuric acid, was extracted with ethyl acetate and derivatized to phenyl acetate using acetic anhydride and K 2CO 3 in water. An n-butyl acetate extract was injected into the GC–MS. The linearity ( r 2) of the calibration curve was greater than 0.996. The analytical repeatability, which is expressed as the relative standard deviation, was less than 6.14%, and the recovery was greater than 96.3%. The method detection limit and the limit of quantitation were 8.0 μg/kg and 26 μg/kg, respectively. The proposed method is also applicable to the analysis of other biological tissues for phenol and its analogs, such as pentachlorophenol.

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          Author and article information

          Journal
          1326
          Acta Chromatographica
          Acta Chromatographica
          Akadémiai Kiadó
          2083-5736
          2018
          : 1-4
          Affiliations
          [ 1 ]School of Natural Resources and Environmental Science, Kangwon National University , Chuncheon, Kangwon-do 24341, Republic of Korea
          [ 2 ]Department of Environmental Science, Kangwon National University , Chuncheon, Kangwon-do 24341, Republic of Korea
          [ 3 ] Natural and Human Co., Ltd. , Bugwon-ro, Wonju, Kangwon-do 26424, Republic of Korea
          [ 4 ]Institute of Environmental Research, Kangwon National University , Chuncheon, Kangwon-do 24341, Republic of Korea
          Author notes
          [*]

          Author for correspondence: kimh@ 123456kangwon.ac.kr

          Article
          10.1556/1326.2018.00529
          © 2018 The Author(s)

          This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (https://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted use, distribution, and reproduction in any medium for non-commercial purposes, provided the original author and source are credited, a link to the CC License is provided, and changes - if any - are indicated.

          Counts
          Pages: 4
          Categories
          Short Communication

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