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      Rickettsia amblyommii infecting Amblyomma sculptum in endemic spotted fever area from southeastern Brazil

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          Abstract

          The Rickettsia bacteria include the aetiological agents for the human spotted fever (SF) disease. In the present study, a SF group Rickettsia amblyommii related bacterium was detected in a field collected Amblyomma sculptum ( Amblyomma cajennense species complex) tick from a Brazilian SF endemic site in southeastern Brazil, in the municipality of Juiz de Fora, state of Minas Gerais. Genetic analysis based on genes ompA, ompB and htrA showed that the detected strain, named R. amblyommii str. JF, is related to the species R. amblyommii.

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          Universal and rapid salt-extraction of high quality genomic DNA for PCR-based techniques.

          S. Aljanabi (1997)
          A very simple, fast, universally applicable and reproducible method to extract high quality megabase genomic DNA from different organisms is described. We applied the same method to extract high quality complex genomic DNA from different tissues (wheat, barley, potato, beans, pear and almond leaves as well as fungi, insects and shrimps' fresh tissue) without any modification. The method does not require expensive and environmentally hazardous reagents and equipment. It can be performed even in low technology laboratories. The amount of tissue required by this method is approximately 50-100 mg. The quantity and the quality of the DNA extracted by this method is high enough to perform hundreds of PCR-based reactions and also to be used in other DNA manipulation techniques such as restriction digestion, Southern blot and cloning.
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            Genotypic identification of rickettsiae and estimation of intraspecies sequence divergence for portions of two rickettsial genes.

            R Regnery, C L Spruill, B D Plikaytis (1991)
            DNA sequences from specific genes, amplified by the polymerase chain reaction technique, were used as substrata for nonisotopic restriction endonuclease fragment length polymorphism differentiation of rickettsial species and genotypes. The products amplified using a single pair of oligonucleotide primers (derived from a rickettsial citrate synthase gene sequence) and cleaved with restriction endonucleases were used to differentiate almost all recognized species of rickettsiae. A second set of primers was used for differentiation of all recognized species of closely related spotted fever group rickettsiae. The procedure circumvents many technical obstacles previously associated with identification of rickettsial species. Multiple amplified DNA digest patterns were used to estimate the intraspecies nucleotide sequence divergence for the genes coding for rickettsial citrate synthase and a large antigen-coding gene of the spotted fever group rickettsiae. The estimated relationships deduced from these genotypic data correlate reasonably well with established rickettsial taxonomic schemes.
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              MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance and maximum parsimony methods

              K. TAMURA, D. PETERSON, N Peterson … (2011)
              Article 0 comments Cited 151 times – based on 0 reviews     Review now
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                Author and article information

                Journal
                Journal ID (nlm-ta): Mem Inst Oswaldo Cruz
                Journal ID (iso-abbrev): Mem. Inst. Oswaldo Cruz
                Journal ID (publisher-id): mioc
                Title: Memórias do Instituto Oswaldo Cruz
                Publisher: Instituto Oswaldo Cruz, Ministério da Saúde
                ISSN (Print): 0074-0276
                ISSN (Electronic): 1678-8060
                Publication date (Print and electronic): December 2015
                Publication date (Print): December 2015
                Volume: 110
                Issue: 8
                Pages: 1058-1061
                Affiliations
                [1 ]Universidade Federal de Juiz de Fora, Instituto de Ciências Biológicas, Programa de Pós-Graduação em Ciências Biológicas, Comportamento e Biologia Animal, Juiz de Fora, MG, Brasil
                [2 ]Fundação Oswaldo Cruz, Instituto Oswaldo Cruz, Laboratório de Referência Nacional em Vetores das Riquetsioses, Rio de Janeiro, RJ, Brasil
                [3 ]Universidade Federal do Rio de Janeiro, Instituto de Biologia, Departamento de Genética, Laboratório de Genética Molecular de Eucariontes e Simbiontes, Rio de Janeiro, RJ, Brasil
                [4 ]Fundação Ezequiel Dias, Laboratório Central de Minas Gerais, Serviço deVirologia e Riquetsioses, Belo Horizonte, MG, Brasil
                Author notes
                [+ ] Corresponding author: gsgazeta@ 123456ioc.fiocruz.br

                ECN and VFV contributed equally to this work.

                Article
                DOI: 10.1590/0074-02760150266
                PMC ID: 4708027
                PubMed ID: 26676317
                SO-VID: 81147981-45b8-4655-b7c7-a79c80ad2fd9
                License:

                This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

                History
                Date received : 15 July 2015
                Date accepted : 6 November 2015
                Page count
                Figures: 1, Tables: 0, Equations: 0, References: 33, Pages: 1
                Categories
                Subject: Short Communication

                Keywords: rickettsia amblyommii,amblyomma sculptum,southeastern brazil -,ticks
                Data availability:
                Keywords: rickettsia amblyommii, amblyomma sculptum, southeastern brazil -, ticks

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