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      Molecular cloning and characterization of autophagy-related gene TmATG8 in Listeria-invaded hemocytes of Tenebrio molitor.

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          Abstract

          Macroautophagy (hereinafter called autophagy) is a highly regulated process used by eukaryotic cells to digest portions of the cytoplasm that remodels and recycles nutrients and disposes of unwanted cytoplasmic constituents. Currently 36 autophagy-related genes (ATG) and their homologs have been characterized in yeast and higher eukaryotes, including insects. In the present study, we identified and functionally characterized the immune function of an ATG8 homolog in a coleopteran insect, Tenebrio molitor (TmATG8). The cDNA of TmATG8 comprises of an ORF of 363 bp that encodes a protein of 120 amino acid residues. TmATG8 transcripts are detected in all the developmental stages analyzed. TmAtg8 protein contains a highly conserved C-terminal glycine residue (Gly116) and shows high amino acid sequence identity (98%) to its Tribolium castaneum homolog, TcAtg8. Loss of function of TmATG8 by RNAi led to a significant increase in the mortality rates of T. molitor larvae against Listeria monocytogenes. Unlike dsEGFP-treated control larvae, TmATG8-silenced larvae failed to turn-on autophagy in hemocytes after injection with L. monocytogenes. These data suggest that TmATG8 play a role in mediating autophagy-based clearance of Listeria in T. molitor.

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          Author and article information

          Journal
          Dev. Comp. Immunol.
          Developmental and comparative immunology
          1879-0089
          0145-305X
          Jul 2015
          : 51
          : 1
          Affiliations
          [1 ] Division of Plant Biotechnology, Institute of Environmentally-Friendly Agriculture (IEFA), College of Agriculture and Life Sciences, Chonnam National University, Gwangju, Republic of Korea; Department of Soil Science, Faculty of Agriculture, Sokoine University of Agriculture, P.O. Box 3008, Chuo Kikuu, Morogoro, Tanzania.
          [2 ] Division of Plant Biotechnology, Institute of Environmentally-Friendly Agriculture (IEFA), College of Agriculture and Life Sciences, Chonnam National University, Gwangju, Republic of Korea.
          [3 ] Division of Plant Biotechnology, Institute of Environmentally-Friendly Agriculture (IEFA), College of Agriculture and Life Sciences, Chonnam National University, Gwangju, Republic of Korea; Trident School of Biotech Sciences, Trident Academy of Creative Technology, Chandrasekharpur, Bhubaneswar 751024, Odisha, India.
          [4 ] Department of Life Science and Biotechnology, College of Natural Sciences, Soonchunhyang University, Asan City, Republic of Korea.
          [5 ] Department of Biology Education, Chungbuk National University, 410 Seongbong Road, Seowon-gu, Cheongju, Chungbuk 361-763, Republic of Korea.
          [6 ] Division of Plant Biotechnology, Institute of Environmentally-Friendly Agriculture (IEFA), College of Agriculture and Life Sciences, Chonnam National University, Gwangju, Republic of Korea. Electronic address: hanys@jnu.ac.kr.
          Article
          S0145-305X(15)00040-3
          10.1016/j.dci.2015.02.017
          25727880
          8129066f-322d-492e-902b-53e1324c460a
          Copyright © 2015 Elsevier Ltd. All rights reserved.
          History

          ATG8,Autophagy,Innate immunity,Listeria monocytogenes,RNA interference,Tenebrio molitor

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