For Publishers
DiscoveryMetadataPeer reviewHostingPublishing
For Researchers
JoinPublishReviewCollect
Register
Blog
About
Search
Advanced search
My ScienceOpen
Search
For Publishers
For Researchers
Blog
About
30
views
38
references
 Top references
cited by
13
    
0 reviews
 Review
0
comments
 Comment
0
recommends
+1 Recommend
0 collections
    0
    shares
      279
      similar
       All similar
      • Record: found
      • Abstract: found
      • Article: not found

      Molecular characterization of tetracycline- and quinolone-resistant Aeromonas salmonicida isolated in Korea

      research-article

      Read this article at

      ScienceOpenPublisherPMC
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          The antibiotic resistance of 16 Aeromonas ( A.) salmonicida strains isolated from diseased fish and environmental samples in Korea from 2006 to 2009 were investigated in this study. Tetracycline or quinolone resistance was observed in eight and 16 of the isolates, respectively, based on the measured minimal inhibitory concentrations. Among the tetracycline-resistant strains, seven of the isolates harbored tetA gene and one isolate harbored tetE gene. Additionally, quinolone-resistance determining regions (QRDRs) consisting of the gyrA and parC genes were amplified and sequenced. Among the quinolone-resistant A. salmonicida strains, 15 harbored point mutations in the gyrA codon 83 which were responsible for the corresponding amino acid substitutions of Ser 83→Arg 83 or Ser 83→Asn 83. We detected no point mutations in other QRDRs, such as gyrA codons 87 and 92, and parC codons 80 and 84. Genetic similarity was assessed via pulsed-field gel electrophoresis, and the results indicated high clonality among the Korean antibiotic-resistant strains of A. salmonicida.

          Related collections

          Most cited references38

          • Record: found
          • Abstract: found
          • Book: not found

          Bergey's Manual of Determinative Bacteriology

          John G. Holt (1994)
          Based on the data contained in the four-volume Bergey's Manual of Systematic Bacteriology, BMDB-9 also includes new genera and species, new combinations, and new taxa published through the January 1992 issue of the IJSB. Users will find short general descriptions that encompass all organisms by Groups; shape and size, Gram reaction, other pertinent morphological features, motility and flagella, relations to oxygen, basic type of metabolism, carbon and energy sources, habitat and ecology. BMDB-9 also includes discussions of difficulties in identification, keys or tables to genera and species, genus descriptions, synonyms, other nomenclatural changes, and numerous illustrations.
          Book 0 comments Cited 340 times – based on 0 reviews
            Bookmark
            • Record: found
            • Abstract: found
            • Article: not found

            Multiplex PCR for detection of plasmid-mediated quinolone resistance qnr genes in ESBL-producing enterobacterial isolates.

            Vincent Cattoir, Laurent Poirel, Vincent O. Rotimi … (2007)
            To develop a rapid and reliable single-tube-based PCR technique for detecting simultaneously the plasmid-mediated quinolone resistance qnrA, qnrB and qnrS genes. After multiple alignments, primers were designed to detect known qnr variants (six for qnrA-, six for qnrB- and two for qnrS-like genes). They were used for screening a collection of 64 expanded-spectrum beta-lactamase (ESBL)-producing enterobacterial isolates from Kuwait, collected from 2002 to 2004, as ESBL genes have been often associated with qnr genes. Sequencing was performed to identify qnr and associated ESBL genes. In optimized conditions, all positive controls (used separately or mixed) confirmed the specificity of the PCR primers. Out of 64 isolates, only 3 isolates were positive for a qnrB-like gene (4.7%), whereas no qnrA-like and qnrS-like gene was detected. A qnrB2 gene was detected in an Enterobacter cloacae K34 (SHV-12+) isolate, whereas qnrB1-like (termed qnrB7) and qnrB6-like (termed qnrB8) genes were identified from E. cloacae K37 (SHV-12+) and Citrobacter freundii K70 (VEB-1b+) isolates, respectively. We report here a fast and reliable technique for rapid screening of qnr-positive strains to be used for epidemiological surveys. A low prevalence of Qnr determinants among ESBL-producing Enterobacteriaceae was identified in the study with Kuwaiti isolates.
            Article 0 comments Cited 136 times – based on 0 reviews     Review now
              Bookmark
              • Record: found
              • Abstract: found
              • Article: not found

              Evolving concepts regarding the genus Aeromonas: an expanding Panorama of species, disease presentations, and unanswered questions.

              Benjamin Abbott, J. Janda (1998)
              It has been almost 10 years since a major review on the association of Aeromonas with human disease has been published. During that period the number of valid species in the genus has grown to 14, with a new family (Aeromonadaceae) established to house this genus. Despite this explosion in the number of new genomospecies, only five (Aeromonas hydrophila, A. caviae, A. veronii, A. jandaei, and A. schubertii) are currently recognized as human pathogens. New syndromes attributed to this genus include hemolytic uremic syndrome, burn-associated sepsis, and a variety of respiratory tract infections, including epiglottitis. Convincing evidence suggests that some aeromonads do cause gastroenteritis, but it is presently unclear whether many of the strains isolated from feces are involved in diarrheal disease. Many questions regarding this genus remain unanswered.
              Article 0 comments Cited 99 times – based on 0 reviews     Review now
                Bookmark
                All references

                Author and article information

                Journal
                Journal ID (nlm-ta): J Vet Sci
                Journal ID (publisher-id): JVS
                Title: Journal of Veterinary Science
                Publisher: The Korean Society of Veterinary Science
                ISSN (Print): 1229-845X
                ISSN (Electronic): 1976-555X
                Publication date (Print): March 2011
                Publication date (Electronic): 05 March 2011
                Volume: 12
                Issue: 1
                Pages: 41-48
                Affiliations
                [1 ]Laboratory of Aquatic Animal Medicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 151-742, Korea.
                [2 ]Department of Microbiology, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 151-742, Korea.
                [3 ]Laboratory of Animal Cell Biotechnology, Department of Agricultural Biotechnology, Seoul National University, Seoul 151-742, Korea.
                Author notes
                Corresponding author: Tel: +82-2-880-1282; Fax: +82-2-880-1213, parksec@ 123456snu.ac.kr

                †The first two authors contributed equally to this work.

                Article
                DOI: 10.4142/jvs.2011.12.1.41
                PMC ID: 3053466
                PubMed ID: 21368562
                SO-VID: 817083f6-1696-4251-aeac-7bf13108946a
                Copyright © Copyright © 2011 The Korean Society of Veterinary Science
                History
                Date received : 23 March 2010
                Date accepted : 14 May 2010
                Categories
                Subject: Original Article

                ScienceOpen disciplines: Veterinary medicine
                Keywords: quinolone-resistance determining region,tetracycline-resistance,aeromonas salmonicida,minimal inhibitory concentration,pulsed-field gel electrophoresis
                Data availability:
                ScienceOpen disciplines: Veterinary medicine
                Keywords: quinolone-resistance determining region, tetracycline-resistance, aeromonas salmonicida, minimal inhibitory concentration, pulsed-field gel electrophoresis

                Comments

                Comment on this article

                scite_

                Similar content279

                See all similar

                Cited by13

                See all cited by

                Most referenced authors367

                See all reference authors