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      Experiencia en intercomparación de parámetros hematológicos empleando sangre fresca

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          Abstract

          Se practicó una dinámica de evaluación externa experimental en hematología, empleando sangre fresca sin preservantes. Fue corroborada su homogeneidad y bioseguridad, transportada en cadena de frío a 33 laboratorios clínicos del Área Metropolitana de Caracas y establecidos los valores de referencia en el laboratorio organizador. Los coeficientes de variación encontrados fueron para hemoglobina (Hb) 5,1 %, hematocrito (Hto) 5,4 %, recuento de glóbulos blancos (GB) 8,7 %, recuento de glóbulos rojos (GR) 4,3 %, recuento de plaquetas (PLQ) 8,0 %, VCM 3,2 %, CHCM 4,2 % y HCM 4,2 %, considerados satisfactorios. Los Índices de Desviación (ID) obtenidos fueron satisfactorios (ID<2,0) en un 97,0 % para GB, 90,9 % Hb, 66,7 % PLQ, 63,6 % GR, 48,5 % Hto, 27,2 % VCM; e inaceptables (ID>2,0) en un 81,8 % para CHCM y 93,9 % para HCM, posiblemente ligado a problemas de calibración de los instrumentos. La sangre fresca constituye una alternativa práctica y económica que puede impulsar la realización de programas de intercomparación a nivel local.

          Translated abstract

          A dynamics of experimental external evaluation in hematology was carried out, employing fresh blood without preservatives. It was corroborated its homogeneity and biosafety, it was transported in chain of cold to the 33 participating clinical laboratories and established the reference values in our laboratory. The coefficient of variation found were: 5.1 % Hemoglobin (HB), 5.4 % haematocrit (HTO), 8.7 % white blood cells (WBC), 4.2 % red blood cells (RBC), 8.0 % platelets (PLT), 3.2 % MCV, 4.2 % MCHC and 4,3 % MHC, all considered satisfactory. The deviation index (DI) obtained were: satisfactory (DI<2.0) 97.0 % WBC, 90.9 % HB, 66.7 % PLT, 63.6 % RBC, 48.5 % HTO, 27.2 % MCV; unsatisfactory (DI>2.0) 81.8 % MCHC and 93.9 % MHC, this probably related to calibration problems of the instruments. Fresh blood constitutes a practical and economic alternative that can impulse the realization of intercomparison programs at local level.

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          Most cited references16

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          Recommendations of the International Council for Standardization in Haematology for Ethylenediaminetetraacetic Acid Anticoagulation of Blood for Blood Cell Counting and Sizing. International Council for Standardization in Haematology: Expert Panel on Cytometry.

          (1993)
          Of the three ethylenediaminetetraacetic acid (EDTA) salts used for anticoagulation of blood specimens for hematologic testing, potassium salts are the most readily soluble. Tripotassium EDTA is dispensed as a liquid and thus causes a slight dilution of the specimen. This salt also has been shown to affect the red blood cell size more at increased concentrations and on storage than the dipotassium salt. Therefore, dipotassium EDTA is recommended as the anticoagulant of choice in specimen collection for blood cell counting and sizing. The amount of dipotassium EDTA used is 1.5-2.2 mg (3.7-5.4 mumol) per milliliter of blood.
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            The WHO International External Quality Assessment Scheme for haematology

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              Requirements and guidance for external quality assessment schemes for health laboratories

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                Author and article information

                Journal
                rfm
                Revista de la Facultad de Medicina
                RFM
                Universidad Central de Venezuela. Facultad de Medicina. Comisión de Publicaciones de la Facultad de Medicina (Caracas, Distrito Capital, Venezuela )
                0798-0469
                June 2007
                : 30
                : 1
                : 50-54
                Affiliations
                [01] orgnameUCV orgdiv1Facultad de Medicina orgdiv2Escuela de Bioanálisis
                [02] orgnameUCV orgdiv1Facultad de Medicina orgdiv2Escuela de Bioanálisis
                [03] orgnameUCV orgdiv1Facultad de Medicina orgdiv2Escuela de Bioanálisis
                Article
                S0798-04692007000100008 S0798-0469(07)03000108
                81f849e0-929e-4128-b66b-acf48ba1a237

                http://creativecommons.org/licenses/by/4.0/

                History
                : 14 November 2006
                : 30 January 2007
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 14, Pages: 5
                Product

                SciELO Venezuela

                Categories
                Trabajos Originales

                Intercomparison,Control de calidad,Sangre fresca,Parámetros hematológicos,Evaluación externa,Intercomparación,Quality control,Fresh blood sample,Hematology tests,External evaluation

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