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      Label-free quantitative proteomic analysis of reserpine-induced depression in mice intervened by berberine.

      1 , 1 , 1
      Pakistan journal of pharmaceutical sciences

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          Abstract

          Proteomic analysis of reserpine-induced depression and the effects of berberine on this were investigated to delineate the possible underlying mechanism. Reserpine was used for the model of behavioral depression. Model mice were treated with berberine. Mice brain proteomic analysis was carried out by label-free nano LC-ESI-OrbiTrap MS/MS technology. The data were processed by Maxquant software. The differentially-expressed proteins were evaluated on GO and KEGG analysis, and key protein expression was validated by Western blot analysis. A total of 278 differentially-expressed proteins were identified. Reserpine could cause cerebral injury and depressive disorder in mice, the mechanism of which is related to steroid hormone biosynthesis, chemical carcinogenesis, nucleotide excision repair and the retinoic acid-inducible gene I-like (RIG-I-like) receptor signaling. Berberine treatments involve 3 distinct proteins in the RIG-I-like receptor signaling. RIG-I was validated, which was over-expressed in the model group and negative in the normal and administration groups. RIG-I mediated neuroinflammation could participate in the process of depression and RIG-I may become a target for berberine against depression.

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          Author and article information

          Journal
          Pak J Pharm Sci
          Pakistan journal of pharmaceutical sciences
          1011-601X
          1011-601X
          Jan 2022
          : 35
          : 1
          Affiliations
          [1 ] Jiangsu Plant Medicine Research and Development Center, Nanjing University of Chinese Medicine, Nanjing, China.
          Article
          35221284
          822508cf-5717-4c0d-987c-30a2214eaaa3
          History

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