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      Isolation of Growth Hormone-Releasing Hormone and Its Receptor Genes from Scatophagus argus and Their Expression Analyses

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          Abstract

          The teleost Scatophagus argus is a species whose females grows faster than males. Growth hormone ( gh) mRNA abundance in females pituitary is higher than that in males; however the mechanism underlining such differential is still unknown. Growth hormone (GH) is tightly associated with GH-releasing hormone (Ghrh) in vertebrates. In this study, Ghrh gene ( ghrh) and its receptor gene, ghrhr, were isolated from S. argus. Tissue expression analysis showed that ghrh and ghrhr were mainly expressed in hypothalamus while ghrhr was expressed in pituitary and gh was predominantly expressed in pituitary. Twenty cultured S. argus individuals were used to compare ghrh, ghrhr and gh mRNA abundances, 120 g and 181 g average weight for male ( n =11) and female ( n = 9), respectively. Real-time PCR indicated that the ghrh and ghrhr mRNA abundances in male hypothalamus were significantly higher than those in female hypothalamus while that of gh mRNA abundance was significantly higher in female pituitary than in male pituitary. The ghrh and ghrhr mRNA abundances were significantly up-regulated in female hypothalamus 3 h after injection of 0.1 mg kg −1 body weight Ghrh while pituitary ghrhr and gh mRNA abundances were not affected. In female hypothalamus, ghrh and ghrhr mRNA abundances were not affected at 6 h post-injection of 4 mg kg −1 body weight 17α-methyltes-tosterone (17α-MT) or 17β-Es-tradiol (E 2). In female pituitary, ghrhr mRNA abundance was down-regulated by 17α-MT while that of gh mRNA abundance was up-regulated by E 2. Our findings indicated that E 2, rather than Ghrh, plays an important role in up-regulating the expression of gh in female S. argus, which should aid to understand the sexual dimorphism of teleost growth.

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          Author and article information

          Journal
          JOUC
          Journal of Ocean University of China
          Science Press and Springer (China )
          1672-5182
          12 November 2019
          01 December 2019
          : 18
          : 6
          : 1486-1496
          Affiliations
          1Guangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species; Key Laboratory of Marine Ecology and Aquaculture Environment of Zhanjiang; Key Laboratory of Aquaculture in South China Sea for Aquatic Economic Animal of Guangdong Higher Education Institutes; Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China
          Author notes
          *Corresponding author: LI Guangli
          Article
          s11802-019-4110-4
          10.1007/s11802-019-4110-4
          Copyright © Ocean University of China, Science Press and Springer-Verlag GmbH Germany 2019.

          The copyright to this article, including any graphic elements therein (e.g. illustrations, charts, moving images), is hereby assigned for good and valuable consideration to the editorial office of Journal of Ocean University of China, Science Press and Springer effective if and when the article is accepted for publication and to the extent assignable if assignability is restricted for by applicable law or regulations (e.g. for U.S. government or crown employees).

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          Self URI (journal-page): https://www.springer.com/journal/11802

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