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      Histopathological Effects of Therapeutic Doses of Combined XO-Inhibitors and ACE-Inhibitors on the Expression of VEGF-A in the Myocardium and Renal Cortex in Chronic Hypertensive Albino Rats

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          Abstract

          Background: Hypertension is risk factor for development of congestive heart failure. The pathogenesis of myocardial and renal cortex changes in hypertension includes structural remodeling and fibrosis. Aim of study is to evaluate the effects of therapeutic doses of combined XO-Inhibitors and ACE-Inhibitors on expression of VEGF-A in the myocardium and renal cortex in chronic hypertensive albino rats. Material & Methods: Thirty male albino rats were divided into: Group I: (control group), Group II (Non-treated Hypertensive rats), Group III (Allopurinol-treated Hypertensive rats), Group IV (Captopril-treated Hypertensive rats) and Group V (Allopurinol-Captopril-treated Hypertensive rats). At 4 and 8 weeks, the rats were anesthetized followed by obtaining of heart and left kidney to be stained with Masson trichrome and Anti-Vascular endothelial growth factor-A antibody. Results: Group II, one month hypertensive rats showed, myocardium showed disarray with significant increase in interstitial fibrosis. The renal cortex showed evidences indicating glomerulosclerosis. Immunohistochemistry, there was high significant decrease in the number of cells of renal cortex with +ve expression of VEGF-A. Later, they showed marked significant increase in interstitial fibrosis. In Group III, myocardium and renal cortex showed high significant increase in interstitial fibrosis. After two month, there were high significant decrease in the surface area of interstitial fibrosis in myocardium and renal cortex with high significant decrease number of the myocardium with +ve expression of VEGF-A. In Group IV, myocardium showed disarray with marked significant reduction in interstitial fibrosis. The renal cortex showed marked significant reduction in the interstitial fibrosis with significant decrease in the number of cells with +ve expression of VEGF-A. Later, myocardium showed the most high marked significant reduction in interstitial fibrosis with highly significant increase in number of cells with positive expression of VEGF-A. In Group V after two month, both myocardium and renal cortex showed nearly normal architecture with marked significant reduction in interstitial fibrosis. Conclusions: Long term therapy with the combination between allopurinol and captopril decreases the fibrotic changes associated with hypertension and enhances the process of angiogenesis.

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          Author and article information

          Journal
          bioRxiv
          July 15 2017
          Article
          10.1101/163824
          82a06ee4-8d94-44dc-9260-8744ff518162
          © 2017
          History

          Forensic science,Ornithology
          Forensic science, Ornithology

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