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      Characterization of brain ecto-apyrase: evidence for only one ecto-apyrase (CD39) gene.

      Brain research. Molecular brain research
      Adenosine Triphosphatases, metabolism, Amino Acid Sequence, Animals, Antigens, CD, genetics, Apyrase, Base Sequence, Brain, enzymology, DNA, Complementary, Gene Expression, Humans, Mice, Molecular Sequence Data, Rats

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          Abstract

          A rat brain cDNA coding for ecto-(Ca,Mg)-apyrase activity was isolated using human CD39 cDNA and functionally expressed in COS-7 cells. The gene codes for a protein with high similarity to human (75% identity) and murine (90% identity) CD39. It is expressed in primary neurons and astrocytes in cell culture as well as in kidney, liver, muscle and spleen. Southern analysis of the mouse genome suggests that there may be a single copy of the ecto-apyrase gene. Interestingly, the human CD39 gene cytologically co-localizes with the susceptibility gene involved in human partial epilepsy with audiogenic symptoms; such a coincidence is consistent with reports on the deficiency of ecto-apyrase activity in the brains of humans with temporal lobe epilepsy and in those of mice with audiogenic seizures.

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