For Publishers
DiscoveryMetadataPeer reviewHostingPublishing
For Researchers
JoinPublishReviewCollect
Register
Blog
About
Search
Advanced search
My ScienceOpen
Search
For Publishers
For Researchers
Blog
About
52
views
56
references
 Top references
cited by
23
    
0 reviews
 Review
0
comments
 Comment
0
recommends
+1 Recommend
0 collections
    0
    shares
      234
      similar
       All similar
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      Salt-Responsive Transcriptome Profiling of Suaeda glauca via RNA Sequencing

      research-article
      Author(s): , , , *
      Editor(s):
      Publication date (Electronic):
      Journal: PLoS ONE
      Publisher: Public Library of Science

      Read this article at

      ScienceOpenPublisherPMC
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Background

          Suaeda glauca, a succulent halophyte of the Chenopodiaceae family, is widely distributed in coastal areas of China. Suaeda glauca is highly resistant to salt and alkali stresses. In the present study, the salt-responsive transcriptome of Suaeda glauca was analyzed to identify genes involved in salt tolerance and study halophilic mechanisms in this halophyte.

          Results

          Illumina HiSeq 2500 was used to sequence cDNA libraries from salt-treated and control samples with three replicates each treatment. De novo assembly of the six transcriptomes identified 75,445 unigenes. A total of 23,901 (31.68%) unigenes were annotated. Compared with transcriptomes from the three salt-treated and three salt-free samples, 231 differentially expressed genes (DEGs) were detected (including 130 up-regulated genes and 101 down-regulated genes), and 195 unigenes were functionally annotated. Based on the Gene Ontology (GO), Clusters of Orthologous Groups (COG) and Kyoto Encyclopedia of Genes and Genomes (KEGG) classifications of the DEGs, more attention should be paid to transcripts associated with signal transduction, transporters, the cell wall and growth, defense metabolism and transcription factors involved in salt tolerance.

          Conclusions

          This report provides a genome-wide transcriptional analysis of a halophyte, Suaeda glauca, under salt stress. Further studies of the genetic basis of salt tolerance in halophytes are warranted.

          Related collections

          Most cited references56

          • Record: found
          • Abstract: found
          • Article: not found

          The Arabidopsis cytochrome P450 CYP707A encodes ABA 8'-hydroxylases: key enzymes in ABA catabolism.

          Tetsuo Kushiro, Masanori Okamoto, Kazumi Nakabayashi … (2004)
          The hormonal action of abscisic acid (ABA) in plants is controlled by the precise balance between its biosynthesis and catabolism. In plants, ABA 8'-hydroxylation is thought to play a predominant role in ABA catabolism. ABA 8'-hydroxylase was shown to be a cytochrome P450 (P450); however, its corresponding gene had not been identified. Through phylogenetic and DNA microarray analyses during seed imbibition, the candidate genes for this enzyme were narrowed down from 272 Arabidopsis P450 genes. These candidate genes were functionally expressed in yeast to reveal that members of the CYP707A family, CYP707A1-CYP707A4, encode ABA 8'-hydroxylases. Expression analyses revealed that CYP707A2 is responsible for the rapid decrease in ABA level during seed imbibition. During drought stress conditions, all CYP707A genes were upregulated, and upon rehydration a significant increase in mRNA level was observed. Consistent with the expression analyses, cyp707a2 mutants exhibited hyperdormancy in seeds and accumulated six-fold greater ABA content than wild type. These results demonstrate that CYP707A family genes play a major regulatory role in controlling the level of ABA in plants.
          Article 0 comments Cited 267 times – based on 0 reviews     Review now
            Bookmark
            • Record: found
            • Abstract: found
            • Article: not found

            Signal transduction during cold, salt, and drought stresses in plants.

            Guo-Tao Huang, Shi-Liang Ma, Li-Ping Bai … (2012)
            Abiotic stresses, especially cold, salinity and drought, are the primary causes of crop loss worldwide. Plant adaptation to environmental stresses is dependent upon the activation of cascades of molecular networks involved in stress perception, signal transduction, and the expression of specific stress-related genes and metabolites. Plants have stress-specific adaptive responses as well as responses which protect the plants from more than one environmental stress. There are multiple stress perception and signaling pathways, some of which are specific, but others may cross-talk at various steps. In this review article, we first expound the general stress signal transduction pathways, and then highlight various aspects of biotic stresses signal transduction networks. On the genetic analysis, many cold induced pathways are activated to protect plants from deleterious effects of cold stress, but till date, most studied pathway is ICE-CBF-COR signaling pathway. The Salt-Overly-Sensitive (SOS) pathway, identified through isolation and study of the sos1, sos2, and sos3 mutants, is essential for maintaining favorable ion ratios in the cytoplasm and for tolerance of salt stress. Both ABA-dependent and -independent signaling pathways appear to be involved in osmotic stress tolerance. ROS play a dual role in the response of plants to abiotic stresses functioning as toxic by-products of stress metabolism, as well as important signal transduction molecules and the ROS signaling networks can control growth, development, and stress response. Finally, we talk about the common regulatory system and cross-talk among biotic stresses, with particular emphasis on the MAPK cascades and the cross-talk between ABA signaling and biotic signaling.
            Article 0 comments Cited 260 times – based on 0 reviews     Review now
              Bookmark
              • Record: found
              • Abstract: found
              • Article: not found

              The cotton WRKY transcription factor GhWRKY17 functions in drought and salt stress in transgenic Nicotiana benthamiana through ABA signaling and the modulation of reactive oxygen species production.

              Huiru Yan, Haihong Jia, XIAOBO CHEN … (2014)
              Drought and high salinity are two major environmental factors that significantly limit the productivity of agricultural crops worldwide. WRKY transcription factors play essential roles in the adaptation of plants to abiotic stresses. However, WRKY genes involved in drought and salt tolerance in cotton (Gossypium hirsutum) are largely unknown. Here, a group IId WRKY gene, GhWRKY17, was isolated and characterized. GhWRKY17 was found to be induced after exposure to drought, salt, H2O2 and ABA. The constitutive expression of GhWRKY17 in Nicotiana benthamiana remarkably reduced plant tolerance to drought and salt stress, as determined through physiological analyses of the germination rate, root growth, survival rate, leaf water loss and Chl content. GhWRKY17 transgenic plants were observed to be more sensitive to ABA-mediated seed germination and root growth. However, overexpressing GhWRKY17 in N. benthamiana impaired ABA-induced stomatal closure. Furthermore, we found that GhWRKY17 modulated the increased sensitivity of plants to drought by reducing the level of ABA, and transcript levels of ABA-inducible genes, including AREB, DREB, NCED, ERD and LEA, were clearly repressed under drought and salt stress conditions. Consistent with the accumulation of reactive oxygen species (ROS), reduced proline contents and enzyme activities, elevated electrolyte leakage and malondialdehyde, and lower expression of ROS-scavenging genes, including APX, CAT and SOD, the GhWRKY17 transgenic plants exhibited reduced tolerance to oxidative stress compared with wild-type plants. These results therefore indicate that GhWRKY17 responds to drought and salt stress through ABA signaling and the regulation of cellular ROS production in plants.
              Article 0 comments Cited 144 times – based on 0 reviews     Review now
                Bookmark
                All references

                Author and article information

                Contributors
                Zhong-Hua Chen: Role: Editor
                Journal
                Journal ID (nlm-ta): PLoS One
                Journal ID (iso-abbrev): PLoS ONE
                Journal ID (publisher-id): plos
                Journal ID (pmc): plosone
                Title: PLoS ONE
                Publisher: Public Library of Science (San Francisco, CA USA )
                ISSN (Electronic): 1932-6203
                Publication date (Electronic): 1 March 2016
                Publication date Collection: 2016
                Volume: 11
                Issue: 3
                Electronic Location Identifier: e0150504
                Affiliations
                [001]Zhejiang Academy of Agricultural Science, Institute of Crops and Nuclear Technology Utilization, Hangzhou Zhejiang 310021, People’s Republic of China
                University of Western Sydney, AUSTRALIA
                Author notes

                Competing Interests: The authors have declared that no competing interests exist.

                Conceived and designed the experiments: HXJ DHZ. Performed the experiments: HXJ DKD QHY DHZ. Analyzed the data: HXJ. Contributed reagents/materials/analysis tools: HXJ. Wrote the paper: HXJ.

                Article
                Publisher ID: PONE-D-15-41818
                DOI: 10.1371/journal.pone.0150504
                PMC ID: 4773115
                PubMed ID: 26930632
                SO-VID: 833d5c36-3c8b-4485-8742-55bec61546ba
                Copyright © © 2016 Jin et al
                License:

                This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

                History
                Date received : 21 September 2015
                Date accepted : 15 February 2016
                Page count
                Figures: 5, Tables: 1, Pages: 14
                Funding
                This work was supported by Genetically Modified Organisms Breeding Major Projects of China (Grant Number: 2014ZX0800403B). Danhua Zhu had a role in study design and preparation of the manuscript.
                Categories
                Subject: Research Article
                Subject: Biology and Life Sciences
                Subject: Plant Science
                Subject: Plant Physiology
                Subject: Plant Defenses
                Subject: Plant Resistance to Abiotic Stress
                Subject: Biology and Life Sciences
                Subject: Plant Science
                Subject: Plant Pathology
                Subject: Plant Resistance to Abiotic Stress
                Subject: Biology and Life Sciences
                Subject: Ecology
                Subject: Plant Ecology
                Subject: Plant-Environment Interactions
                Subject: Plant Resistance to Abiotic Stress
                Subject: Ecology and Environmental Sciences
                Subject: Ecology
                Subject: Plant Ecology
                Subject: Plant-Environment Interactions
                Subject: Plant Resistance to Abiotic Stress
                Subject: Biology and Life Sciences
                Subject: Plant Science
                Subject: Plant Ecology
                Subject: Plant-Environment Interactions
                Subject: Plant Resistance to Abiotic Stress
                Subject: Physical Sciences
                Subject: Chemistry
                Subject: Chemical Properties
                Subject: Salinity
                Subject: Physical Sciences
                Subject: Chemistry
                Subject: Physical Chemistry
                Subject: Chemical Properties
                Subject: Salinity
                Subject: Biology and life sciences
                Subject: Biochemistry
                Subject: Proteins
                Subject: DNA-binding proteins
                Subject: Transcription Factors
                Subject: Biology and Life Sciences
                Subject: Genetics
                Subject: Gene Expression
                Subject: Gene Regulation
                Subject: Transcription Factors
                Subject: Biology and Life Sciences
                Subject: Biochemistry
                Subject: Proteins
                Subject: Regulatory Proteins
                Subject: Transcription Factors
                Subject: Biology and Life Sciences
                Subject: Organisms
                Subject: Plants
                Subject: Seedlings
                Subject: Biology and Life Sciences
                Subject: Computational Biology
                Subject: Genome Analysis
                Subject: Transcriptome Analysis
                Subject: Biology and Life Sciences
                Subject: Genetics
                Subject: Genomics
                Subject: Genome Analysis
                Subject: Transcriptome Analysis
                Subject: Biology and Life Sciences
                Subject: Cell Biology
                Subject: Cellular Structures and Organelles
                Subject: Cell Walls
                Subject: Plant Cell Walls
                Subject: Biology and Life Sciences
                Subject: Cell Biology
                Subject: Cellular Types
                Subject: Plant Cells
                Subject: Plant Cell Walls
                Subject: Biology and Life Sciences
                Subject: Cell Biology
                Subject: Plant Cell Biology
                Subject: Plant Cells
                Subject: Plant Cell Walls
                Subject: Biology and Life Sciences
                Subject: Plant Science
                Subject: Plant Cell Biology
                Subject: Plant Cells
                Subject: Plant Cell Walls
                Subject: Biology and Life Sciences
                Subject: Organisms
                Subject: Plants
                Subject: Brassica
                Subject: Arabidopsis Thaliana
                Subject: Research and Analysis Methods
                Subject: Model Organisms
                Subject: Plant and Algal Models
                Subject: Arabidopsis Thaliana
                Subject: Biology and life sciences
                Subject: Molecular biology
                Subject: Molecular biology techniques
                Subject: Sequencing techniques
                Subject: RNA sequencing
                Subject: Research and analysis methods
                Subject: Molecular biology techniques
                Subject: Sequencing techniques
                Subject: RNA sequencing
                Custom metadata
                Data Availability All raw data files are available from the SRA database (accession number PRJNA295637).

                ScienceOpen disciplines: Uncategorized
                Data availability:
                ScienceOpen disciplines: Uncategorized

                Comments

                Comment on this article

                scite_

                Similar content234

                See all similar

                Cited by22

                See all cited by

                Most referenced authors746

                See all reference authors