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      A photoactivatable GFP for selective photolabeling of proteins and cells.

      Science (New York, N.Y.)
      American Association for the Advancement of Science (AAAS)

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          Abstract

          We report a photoactivatable variant of the Aequorea victoria green fluorescent protein (GFP) that, after intense irradiation with 413-nanometer light, increases fluorescence 100 times when excited by 488-nanometer light and remains stable for days under aerobic conditions. These characteristics offer a new tool for exploring intracellular protein dynamics by tracking photoactivated molecules that are the only visible GFPs in the cell. Here, we use the photoactivatable GFP both as a free protein to measure protein diffusion across the nuclear envelope and as a chimera with a lysosomal membrane protein to demonstrate rapid interlysosomal membrane exchange.

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          Journal
          12228718
          10.1126/science.1074952

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