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      Localization capability and limitation of electron-multiplying charge-coupled, scientific complementary metal-oxide semiconductor, and charge-coupled devices for superresolution imaging.

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          Abstract

          Localization of a single fluorescent molecule is required in a number of superresolution imaging techniques for visualizing biological structures at cellular and subcellular levels. The localization capability and limitation of low-light detectors are critical for such a purpose. We present an updated evaluation on the performance of three typical low-light detectors, including a popular electron-multiplying CCD (EMCCD), a newly developed scientific CMOS (sCMOS), and a representative cooled CCD, for superresolution imaging. We find that under some experimental accessible conditions, the sCMOS camera shows a competitive and even better performance than the EMCCD camera, which has long been considered the detector of choice in the field of superresolution imaging.

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          Author and article information

          Journal
          J Biomed Opt
          Journal of biomedical optics
          1560-2281
          1083-3668
          : 15
          : 6
          Affiliations
          [1 ] Huazhong University of Science and Technology,, Wuhan National Laboratory for Optoelectronics, Wuhan 430074, China.
          Article
          10.1117/1.3505017
          21198179
          8433b539-46b0-4e51-919d-162d7550c6fe
          History

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