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Characterization of the Protein Subset Desorbed by MALDI from Whole Bacterial Cells
February 2001
February 2001
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Abstract
This study characterizes various features of the proteins that are detected in MALDI
mass spectra when whole bacteria cells are analyzed, in an effort to understand why
some proteins are successfully detected and many others are not. Forty peaks observed
in the mass range 4,000-20,000 Da in the spectra of Escherichia coli K-12 and 11775
are tentatively assigned to proteins in a protein database, and these proteins are
characterized by cell location, copy number, pI, and hydropathicity. Those detected
originate in the cytosol and generally share the traits of high abundance within the
cell, strong bacisity, and medium hydrophilicity.