Pruning is one of the most crucial tree maintenance activities which give an impact on the tree's health and structure. Besides, improper pruning will contribute to the risk of injury to property and the public. This study aims to assess pruning knowledge among four Local authorities in Malaysia. Results found that 69.3 percent of tree pruning workers have a Good pruning knowledge level. However, Topping, pruning types and pruning cut dimension shows the lowest mean percentage of the correct answer. The findings also show that there is a significant positive relationship between pruning knowledge and education level and frequency attending pruning courses.