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      Preparation and Development of Solid Phase Radioimmunoassay System for Determination of Cortisol.

      1
      Journal of immunoassay & immunochemistry
      Informa UK Limited
      125I, Chloramin-T, Coated tubes, Cortisol, HPLC, RIA, Solid phase

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          Abstract

          The goal of the present work was oriented to prepare stable polystyrene coated tubes for direct radioimmunoassay (RIA) of human cortisol. Coating process was performed using rabbit polyclonal antisera specific for cortisol. The stability study showed that these tubes could be stored for up to one year at 4 ºC without any appreciable reduction in their binding. Labeling of cortisol was carried out using chloramin-T method and the (125)I-cortisol tracer was purified using HPLC column. Preparation of polyclonal antibodies (anti-cortisol) was carried out in host rabbit animals against cortisol-21-hemisuccinate:Bovine serum albumin conjugate. The statistical analysis revealed good correlations between the results from the present study and the commercial IZOTOP RIA kit. The local may be extremely helpful in diagnosis and proper management of adrenal cortex disorders.

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          Author and article information

          Journal
          J Immunoassay Immunochem
          Journal of immunoassay & immunochemistry
          Informa UK Limited
          1532-4230
          1532-1819
          May 24 2017
          Affiliations
          [1 ] a Labeled Compounds Dept., Hot Labs Center , Egyptian Atomic Energy Authority , Cairo , Egypt .
          Article
          10.1080/15321819.2017.1331169
          28537478
          8475c3e2-6551-4e87-b296-6e0baf145d6c

          125I,Chloramin-T,Coated tubes,Cortisol,HPLC,RIA,Solid phase
          125I, Chloramin-T, Coated tubes, Cortisol, HPLC, RIA, Solid phase

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