Fermentation optimization of rennet-producing Bacillus amyloliquefaciens GSBa-1 for high-density culture and its kinetic model

There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

Abstract

Abstract The milk-clotting enzyme (MCE) produced by Bacillus amyloliquefaciens GSBa-1 achieved the scale-up criterion from 0.25-L erlenmeyer flask to 5-L bioreactor sucessfully and exhibited remarkable milk-clotting activity (MCA) (626.3 SU/mL) that was 1.45-fold higher than the control by optimizing both medium composition and fermentation conditions.The growth of GSBa-1 was optimized through response surface methodology employing Plackett-Burman design and Box-Behnken design. The final optimized fermentation conditions were as follows: maltose (7.258g/L), corn steep liquor (8.15g/L), and Tween 80 (1.97g/L); constant fermentation pH (7.0), temperature (45 °C) and stirrer speed (200 rpm). Kinetic models of microbial growth (X), product formation (P), and substrate consumption (S) were constructed, respectively. The derived model was the first reported model for GSBa-1 rennet production, contributing visual description for the rennet fermentation process, as well as the improvement of its productivity and efficiency.

Related collections

Most cited references 45

Record: found
Abstract: not found
Article: not found

THE DESIGN OF OPTIMUM MULTIFACTORIAL EXPERIMENTS

R. L. PLACKETT, J. BURMAN (1946)

0 comments Cited 403 times – based on 0 reviews      Review now

Record: found
Abstract: found
Article: not found

Prebiotic, Probiotic, Antimicrobial, and Functional Food Applications of Bacillus amyloliquefaciens.

Kalekristos WoldemariamYohannes, Zhen Wan, Qinglin Yu … (2020)

Bacillus amyloliquefaciens belongs to the genus Bacillus and family Baciliaceae. It is ubiquitously found in food, plants, animals, soil, and in different environments. In this review, the application of B. amyloliquefaciens in probiotic and prebiotic microbes in fermentation, synthesis, and hydrolysis of food compounds is discussed as well as further insights into its potential application and gaps. B. amyloliquefaciens is also a potential microbe in the synthesis of bioactive compounds including peptides and exopolysaccharides. In addition, it can synthesize antimicrobial compounds (e.g., Fengycin, and Bacillomycin Lb), which makes its novelty in the food sector greater. Moreover, it imparts and improves the functional, sensory, and shelf life of the end products. The hydrolysis of complex compounds including insoluble proteins, carbohydrates, fibers, hemicellulose, and lignans also shows that B. amyloliquefaciens is a multifunctional and potential microbe which can be applied in the food industry and in functional food processing.

0 comments Cited 38 times – based on 0 reviews      Review now

Record: found
Abstract: found
Article: not found

Fed-batch high-cell-density fermentation strategies for Pichia pastoris growth and production.

Wan-Cang Liu, Sarah Inwood, Ting Gong … (2019)

Pichia pastoris is extensively used to produce various heterologous proteins. Amounts of biopharmaceutical drugs and industrial enzymes have been successfully produced by fed-batch high-cell-density fermentation (HCDF) of this cell factory. High levels of cell mass in defined media can be easily achieved and therefore large quantities of recombinant proteins with enhanced activities and lower costs can be obtained through HCDF technology. A robust HCDF process makes a successful transition to commercial production. Recently, efforts have been made to increase the heterologous protein production and activity by the HCDF of P. pastoris. However, challenges around selecting a suitable HCDF strategy exist. The high-level expression of a specific protein in P. pastoris is still, at least in part, limited by optimizing the methanol feeding strategy. Here, we review the progress in developments and applications of P. pastoris HCDF strategies for enhanced expression of recombinant proteins. We focus on the methanol induction strategies for efficient fed-batch HCDF in bioreactors, mainly focusing on various stat-induction strategies, co-feeding, and the limited induction strategy. These processes control strategies have opened the door for expressing foreign proteins in P. pastoris and are expected to enhance the production of recombinant proteins.

0 comments Cited 34 times – based on 0 reviews      Review now

All references

Author and article information

Journal

Journal ID (publisher-id): cta

Title: Food Science and Technology

Abbreviated Title: Food Sci. Technol

Publisher: Sociedade Brasileira de Ciência e Tecnologia de Alimentos (Campinas, SP, Brazil )

ISSN (Print): 0101-2061

ISSN (Electronic): 1678-457X

Publication date (Print and electronic): 2022

Volume: 42

Electronic Location Identifier: e40122

Affiliations

[01] Beijing orgnameBeijing Technology and Business University orgdiv1Beijing Engineering and Technology Research Center of Food Additives orgdiv2Beijing Advanced Innovation Center for Food Nutrition and Human Health P. R. China

Article

Publisher ID: S0101-20612022000101179 Publisher ID: S0101-2061(22)04200001179

DOI: 10.1590/fst.40122

SO-VID: 84ac2863-f9a9-4a8a-900a-37fed963f0be

License:

This work is licensed under a Creative Commons Attribution 4.0 International License.

History

Date accepted : 06 May 2022

Date received : 22 March 2022

Page count

Figures: 0, Tables: 0, Equations: 0, References: 45, Pages: 0

Product

SciELO Brazil