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      The Glomerular Polyanion (GPA) of the Rat Kidney

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          Abstract

          Rat peritoneal exudate cells (PEC) or human peripheral blood cells stimulated with mitogens in vitro were cultured in tissue culture chambers mounted on rat or human cryostat kidney sections. After 20 h, the cultures were discontinued and the glomerular polyanion (GPA) of the glomeruli was studied using the colloidal iron stain. The results show that in contrast to PHA-stimulated cells, rat PEC or human blood cells stimulated with Con-A are able to reduce GPA stainability. It was further shown that rat PEC activated with Con-A for 20 or 48 h were able to suppress in vitro lymphocyte transformation of syngeneic blood lymphocytes in a co-culture system following mitogenic stimulation. In view of recent concepts according to which disturbance of T cell function is associated with in vivo loss of GPA in some forms of the nephrotic state, we conclude that investigation into the possible in vivo significance of the present observations is worthwhile.

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          Author and article information

          Journal
          NEF
          Nephron
          10.1159/issn.1660-8151
          Nephron
          S. Karger AG
          1660-8151
          2235-3186
          1982
          1982
          03 December 2008
          : 31
          : 1
          : 68-74
          Affiliations
          Section of Immunopathology, Department of Pathology, State University of Groningen, The Netherlands
          Article
          182620 Nephron 1982;31:68–74
          10.1159/000182620
          6213877
          © 1982 S. Karger AG, Basel

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          Page count
          Pages: 7
          Categories
          Original Paper

          Cardiovascular Medicine, Nephrology

          Co-cultures, Suppressor cells in vitro, Glomerular polyanion

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