BACKGROUND: Despite the increasing body of research on workplace incivility, the relationship between co-worker/supervisor incivilities and job-affective well-being have received relatively little consideration from the scholars. This study investigates the impact of polychronicity on nurses’ psychological well-being in the healthcare sector. OBJECTIVE: According to COR theory, this study’s main objective is to investigate the impact of two aspects of incivilities, co-worker, and supervisor incivilities, as the moderating effect on the relationship between polychronicity and job-affective psychological well-being. METHODS: The sample data was collected from 260 nurse’s staff who were working in hospitals. The proposed model hypothesis was tested through SPSS-Amos-structural equation modeling. RESULTS: The result shows that polychronic nurses show high job engagement and job performance in a hospital environment. This study’s findings revealed that co-workers and supervisors’ higher incivility weakens the positive relationship between polychronicity and job-affective well-being. CONCLUSION: Today, retained the employees are a perilous issue in every organization, especially in hospital sectors, where nurses are working in stressful working environments, facing bullying, harassment, and uncivil behavior from patients’ side, co-workers, and supervisor. These types of behaviors are not only affecting employees’ well-being, commitment, and job performance but also their mental health and intent to leave. Similarly, the effect on organizational goals, shareholders’ value, and progress makes an effective mechanism to control the negative behaviors that would be an apparent advantage for administration and managers to efficiently attain organizational goals.