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      Estradiol and proinflammatory cytokines stimulate ISG20 expression in synovial fibroblasts of patients with osteoarthritis

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          Summary

          Interferon stimulated gene 20-kDa (ISG20) has been implicated in the pathology of osteoarthritis (OA) and it has been separately found to be responsive to estrogen stimulation. OA disproportionately affects women, and especially older women, suggesting some role of reproductive hormones in its pathology. The current study characterized the expression of ISG20 following stimulation with estradiol (E2) and proinflammatory cytokines in OA synovial fibroblasts (OASFs). E2 and the proinflammatory cytokines interleukin-6 (IL-6), lipopolysaccharide (LPS), and tumor necrosis factor α (TNF-α) were used to stimulate OASFs in vitro. The expression of ISG20 before and after stimulation was detected using quantitative real-time polymerase chain reaction (RT-qPCR) and Western blotting. E2 and proinflammatory cytokine (IL-6, LPS and TNF-α) stimulation significantly induced the expression of ISG20 both at the messenger RNA (mRNA) and protein level. Moreover, the induction was time- and dose-dependent. Small interfering RNA (siRNA) was transfected into OASFs, and expression of the inflammatory factors interleukin-1α (IL-1α), IL-6, and interleukin-10 (IL-10) was detected using RT-qPCR. Silencing ISG20 with siRNA inhibited the expression of IL-1α, IL-6, and IL-10. Thus, expression of ISG20 was regulated by estradiol and proinflammatory factors, while ISG20 in turn regulated the expression of other inflammatory factors. These data support the contention that ISG20 plays a role in the inflammatory process of OA.

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          Author and article information

          Journal
          Intractable Rare Dis Res
          Intractable Rare Dis Res
          irdr
          irdr
          Intractable & Rare Diseases Research
          International Research and Cooperation Association for Bio & Socio-Sciences Advancement
          2186-3644
          2186-361X
          November 2017
          : 6
          : 4
          : 269-273
          Affiliations
          [1 ] School of Medicine and Life Sciences, University of Jinan-Shandong Academy of Medical Sciences, Ji'nan, China
          [2 ] Shandong Medicinal Biotechnology Center, Ji'nan, China
          [3 ] Key Laboratory for Rare & Uncommon Diseases of Shandong Province, Ji'nan, China
          [4 ] Key Laboratory for Biotech-drugs of the Ministry of Health, Ji'nan, China
          Author notes
          [* ] Address correspondence to: Dr. Jihong Pan, Shandong Medicinal and Biotechnology Center, Shandong Academy of Medical Sciences, 18877 Jingshi Road, Ji'nan, Shandong 250062, China. E-mail: pjh933@ 123456sohu.com
          Article
          PMC5735280 PMC5735280 5735280
          10.5582/irdr.2017.01062
          5735280
          29259855
          85928a9b-5dc1-4c2e-8016-18a1746b27b5
          2017, International Research and Cooperation Association for Bio & Socio-Sciences Advancement
          History
          : 13 September 2017
          : 16 October 2017
          : 24 October 2017
          Page count
          Figures: 3, Tables: 0, References: 16, Pages: 5
          Categories
          Original Article

          inflammation,proinflammatory factor,Estradiol,ISG20,Osteoarthritis

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