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      Influence of Incubation Temperature on 9,10-Anthraquinone-2-Sulfonate (AQS)-Mediated Extracellular Electron Transfer

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          The electron shuttling process has been recognized as an important microbial respiration process. Because the incubation temperature can influence both the reactivity of electron mediators and cell growth, it may also affect the electron-shuttle-mediated extracellular electron transfer (EET) process. Here, the effect of incubation temperature (22–38°C) was investigated in a bioelectrochemical system (BES) using Shewanella oneidensis MR-1 and 50 μM of 9,10-anthraquinone-2-sulfonate (AQS). We found that current generation increased as the temperature was increased from 22 to 34°C and then decreased sharply at 38°C. The biofilm biomass, as indicated by the total protein extracted from the electrode, increased as the temperature increased from 22 to 34°C and then decreased at 38°C, mirroring the current generation results. These results were further confirmed by increasing the temperature slowly, step-by-step, in a single BES with a constant biofilm biomass, suggesting that the EET rates could be substantially influenced by temperature, even with the same biofilm. The effects of temperature on the AQS bioreduction rate, c-type cytochrome ( c-Cyts)-bound-cofactor-mediated EET, the AQS mid-point potential, and the AQS diffusion coefficient were studied. From these results, we were able to conclude that temperature influenced the EET rates by changing the c-Cyts-bound-cofactor-mediated EET process and the AQS bioreduction rate, and that the change in biofilm formation was a dominant factor influencing the overall EET rates. These findings should contribute to the fundamental understanding of EET processes. Moreover, optimization of the operating parameters for current generation will be helpful for the practical application of bioelectrochemical techniques.

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          Most cited references 42

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          Exoelectrogenic bacteria that power microbial fuel cells.

           Bruce Logan (2009)
          There has been an increase in recent years in the number of reports of microorganisms that can generate electrical current in microbial fuel cells. Although many new strains have been identified, few strains individually produce power densities as high as strains from mixed communities. Enriched anodic biofilms have generated power densities as high as 6.9 W per m(2) (projected anode area), and therefore are approaching theoretical limits. To understand bacterial versatility in mechanisms used for current generation, this Progress article explores the underlying reasons for exocellular electron transfer, including cellular respiration and possible cell-cell communication.
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            Bacterial manganese reduction and growth with manganese oxide as the sole electron acceptor.

            Microbes that couple growth to the reduction of manganese could play an important role in the biogeochemistry of certain anaerobic environments. Such a bacterium, Alteromonas putrefaciens MR-1, couples its growth to the reduction of manganese oxides only under anaerobic conditions. The characteristics of this reduction are consistent with a biological, and not an indirect chemical, reduction of manganese, which suggest that this bacterium uses manganic oxide as a terminal electron acceptor. It can also utilize a large number of other compounds as terminal electron acceptors; this versatility could provide a distinct advantage in environments where electron-acceptor concentrations may vary.
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              Anaerobic production of magnetite by a dissimilatory iron-reducing microorganism


                Author and article information

                1College of Materials and Energy, South China Agricultural University , Guangzhou, China
                2Guangdong Key Laboratory of Integrated Agro-Environmental Pollution Control and Management, Guangdong Institute of Eco-Environmental Science and Technology , Guangzhou, China
                Author notes

                Edited by: Fanghua Liu, Yantai Institute of Coastal Zone Research (CAS), China

                Reviewed by: Souichiro Kato, National Institute of Advanced Industrial Science and Technology (AIST), Japan; Liang Shi, China University of Geosciences, China

                *Correspondence: Tongxu Liu, txliu@

                These authors have contributed equally to this work

                This article was submitted to Microbiotechnology, Ecotoxicology and Bioremediation, a section of the journal Frontiers in Microbiology

                Front Microbiol
                Front Microbiol
                Front. Microbiol.
                Frontiers in Microbiology
                Frontiers Media S.A.
                06 March 2019
                : 10
                6414795 10.3389/fmicb.2019.00464
                Copyright © 2019 Liu, Wu, Liu, Li, Dong and Jing.

                This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

                Figures: 8, Tables: 0, Equations: 0, References: 42, Pages: 10, Words: 0
                Funded by: National Natural Science Foundation of China 10.13039/501100001809
                Award ID: 41701305
                Award ID: 41522105
                Award ID: 21701045
                Original Research


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