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Abstract
Eighteen cDNAs, cloned from interferon-treated T98G neuroblastoma cells, correspond
to seven different mRNAs induced up to 40-fold by interferon. One codes for metallothionein
II and another for a class I HLA. The others do not code for proteins of known sequence.
In the continued presence of interferon, accumulation of the mRNAs continues for about
1 day but ceases whenever interferon is removed. Once induced, the mRNAs are stable.
Synthesis of new proteins is not required for induction. The rate of transcription
of one of the genes doubles 5 min after treatment with interferon and reaches a maximum
by 60 min. This rate begins to fall after 4-6 hr, reaching the uninduced level by
8-12 hr. Since the mRNA continues to accumulate after 8-12 hr, posttranscriptional
events must also play a role in increasing its level.
A rapid, direct method for screening single plaques of Agt recombinant phage is described. The method allows at least 10(6) clones to be screened per day and simplifies physical containment of recombinants.