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Transcriptional and posttranscriptional regulation of interferon-induced gene expression in human cells.

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genetics, Cell Line, Cell Nucleus, metabolism, Cloning, Molecular, Cycloheximide, pharmacology, DNA, Genes, drug effects, HLA Antigens, Animals, Interferon Type I, Kinetics, Metallothionein, Mice, Neuroblastoma, Nucleic Acid Hybridization, RNA Processing, Post-Transcriptional, RNA, Messenger, Transcription, Genetic

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      Abstract

      Eighteen cDNAs, cloned from interferon-treated T98G neuroblastoma cells, correspond to seven different mRNAs induced up to 40-fold by interferon. One codes for metallothionein II and another for a class I HLA. The others do not code for proteins of known sequence. In the continued presence of interferon, accumulation of the mRNAs continues for about 1 day but ceases whenever interferon is removed. Once induced, the mRNAs are stable. Synthesis of new proteins is not required for induction. The rate of transcription of one of the genes doubles 5 min after treatment with interferon and reaches a maximum by 60 min. This rate begins to fall after 4-6 hr, reaching the uninduced level by 8-12 hr. Since the mRNA continues to accumulate after 8-12 hr, posttranscriptional events must also play a role in increasing its level.

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      Most cited references 36

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        Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

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          Screening lambdagt recombinant clones by hybridization to single plaques in situ.

          A rapid, direct method for screening single plaques of Agt recombinant phage is described. The method allows at least 10(6) clones to be screened per day and simplifies physical containment of recombinants.
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            6548414

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