Fodinicola acaciae sp. nov., an Endophytic Actinomycete Isolated from the Roots of Acacia mangium Willd. and Its Genome Analysis

Abstract

A novel endophytic actinomycete strain GKU 173 ^T isolated from the roots of Acacia mangium Willd. showed potential plant growth promoting (PGP) activity. Phylogenetic analysis, based on 16S rRNA gene, indicated that strain GKU 173 ^T was the most closely related to Fodinicola feengrottensis HKI 0501 ^T—the only species in the genus Fodinicola. Morphology and chemotaxonomy of strain GKU 173 ^T indicated that it belongs to the genus Fodinicola by having meso-diaminopimelic acid in the cell wall and xylose as the characteristic cell-wall sugars. The cellular fatty acid profile mainly comprised iso-C _16:0, anteiso-C _17:0, iso-C _18:0, and iso-C _17:0. The major menaquinones were MK-9(H ₄), MK-9(H ₆), and MK-9(H ₈). The main polar phospholipids contained diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), and phosphatidylinositol (PI). Genome analysis signified DNA G+C content of 67.81 mol%. The level of digital DNA-DNA relatedness between strain GKU 173 ^T and the type strain was 21.30%. On the basis of polyphasic characteristics, strain GKU 173 ^T clearly represents a novel species of the genus Fodinicola, for which the name Fodinicola acaciae sp. nov. (= TBRC 10620 ^T = NBRC 114213 ^T) is proposed. Furthermore, genome analysis of both strains suggested that members of the genus Fodinicola are promising sources of beneficial PGP-actinomycetes and novel secondary metabolites.