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      Holistic evaluation of San-Huang Tablets using a combination of multi-wavelength quantitative fingerprinting and radical-scavenging assays

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          The present study was designed to establish a multi-wavelength quantitative fingerprinting method for San-Huang Tablets (SHT), a widely used and commercially available herbal preparation, where high performance liquid chromatography (HPLC) with a diode array detector (DAD) was employed to obtain the fingerprint profiles. A simple linear quantitative fingerprint method (SLQFM) coupled with multi-ingredient simultaneous determination was developed to evaluate the quality consistency of the tested samples qualitatively and quantitatively. Additionally, the component–activity relationship between chromatographic fingerprints and total radical-scavenging capacity in vitro (as assessed using the 1, 1-diphenyl-2-picrylhydrazyl (DPPH) assay) was investigated by partial least squares regression (PLSR) analysis to predict the antioxidant capacity of new samples from the chromatographic fingerprints and identify the main active constituents that can be used as the target markers for the quality control of SHT. In conclusion, the strategy developed in the present study was effective and reliable, which can be employed for holistic evaluation and accurate discrimination for the quality consistency of SHT preparations and other traditional Chinese medicine (TCM) and herbal preparations as well.

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          Most cited references 12

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          Development of a novel method combining HPLC fingerprint and multi-ingredients quantitative analysis for quality evaluation of traditional Chinese medicine preparation.

          A novel method combining high performance liquid chromatography (HPLC) fingerprint and simultaneous quantitative analysis of multiple active components was developed and validated for quality evaluation of one type of traditional Chinese medicine preparations: Shuang-huang-lian (SHL) oral liquid formulation. For fingerprint analysis, 45 peaks were selected as the common peaks to evaluate the similarities among several different SHL oral liquid preparations collected from manufacturers. Additionally, simultaneous quantification of eleven markers, including chlorogenic acid, caffeic acid, rutin, forsythiaside, scutellarin, baicalin, forsythin, luteoloside, apigenin, baicalein and wogonin, was performed. Statistical analysis of the obtained data demonstrated that our method has achieved desired linearity, precision and accuracy. Finally, concentrations of these eleven markers in SHL oral liquid prepared by different manufacturers in China were determined. These results demonstrated that the combination of HPLC chromatographic fingerprint and simultaneous quantification of multi-ingredients offers an efficient and reliable approach for quality evaluation of SHL oral liquid preparations. Copyright © 2011 Elsevier B.V. All rights reserved.
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            Combinative method using HPLC fingerprint and quantitative analyses for quality consistency evaluation of an herbal medicinal preparation produced by different manufacturers.

             Yan. Li,  Tao Wu,  Jinghui Zhu (2010)
            A combinative method using HPLC-DAD fingerprint and quantitative analysis was developed and validated for manufacturer-to-manufacturer quality consistency evaluation of Yiqing preparations. For fingerprint analysis, 22 peaks were selected as the characteristic peaks to evaluate the similarities of different samples collected from different manufacturers. The similarities of 12 Yiqing samples were beyond 0.90, indicating that samples from different manufacturers were, to some extent, consistent. Additionally, simultaneous quantification of nine markers including berberine, aloe-emodin, rhein, emodin, chrysophanol, baicalin, baicalein, wogonoside, and wogonin in Yiqing was performed to interpret the quality consistency. The results from the quantitative data showed that the contents of these nine marker compounds were quite consistent for batches produced within one manufacturer and significantly different from manufacturer-to-manufacturer. This study demonstrated that a combination of the chromatographic fingerprint and quantitative analysis offers an efficient way to quality consistency evaluation of herbal preparation. Copyright (c) 2010 Elsevier B.V. All rights reserved.
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              Fingerprint-efficacy study of artificial Calculus bovis in quality control of Chinese materia medica.

              For quality control of Chinese materia medica (CMM), an attempt on fingerprint-efficacy study of artificial Calculus bovis was developed in this work. Chemical fingerprints of artificial C. bovis samples from ten different sources were determined by UPLC-ELSD and investigated by similarity analysis and hierarchical clustering analysis. Antibacterial effects of these samples on Escherichia coli growth were measured using microcalorimetry. The fingerprint-efficacy relationship of chemical fingerprint and antibacterial effect of artificial C. bovis were established by multi-linear regression analysis. Our results showed that the sources and places of production of artificial C. bovis had some important influence on the chemical fingerprints and antibacterial effects of this CMM. These artificial C. bovis could be grouped into four clusters according to their chemical fingerprints and antibacterial effects. Compounds cholic acid, taurocholate sodium, hyodeoxycholic acid and one unknown compound might be the major effective components for quality control of this CMM. Fingerprint-efficacy study provided a powerful way and some insight for the quality control of artificial C. bovis and other CCMs. Crown Copyright © 2011. Published by Elsevier Ltd. All rights reserved.

                Author and article information

                Chinese Journal of Natural Medicines
                20 April 2017
                : 15
                : 4
                : 310-320
                1College of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China
                2College of Pharmacy and Chemistry, Dali University, Dali 671000, China
                3College of Pharmacy, China Pharmaceutical University, Nanjing 210009, China
                4Department of Pharmacy, Traditional Chinese Medicine Hospital of Dali, Dali 671000, China
                Author notes
                *Corresponding author: SUN Guo-Xiang, Tel: 86-24-23986286, E-mail: gxswmwys@

                These authors have no conflict of interest to declare.

                Copyright © 2017 China Pharmaceutical University. Published by Elsevier B.V. All rights reserved.
                Funded by: National Natural Science Foundation of China
                Award ID: 81560695
                Funded by: Doctoral Research Fund of Dali University
                Award ID: KYBS201512
                This work was financially supported by the National Natural Science Foundation of China (No. 81560695), and the Doctoral Research Fund of Dali University (No. KYBS201512).


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