Jéssyca Ketterine Carvalho a , b , * , Andressa Alves Silva Panatta a , c , Maruhen Amir Datsch Silveira d , e , Christophe Tav d , e , Susana Johann f , Maria Luiza Fernandes Rodrigues a , Cleide Viviane Buzanello Martins a
29 May 2021
Yeast isolated from a lotic ecosystem in Brazil show high enzymatic production.
One third of the individuals tested have demostrated the ability to secrete more than one enzyme.
Naganishia diffluens and Apiotrichum mycotoxinivorans show potential applicability as biocatalysts.
Individuals from Apiotrichum mycotoxinivorans were the best producers of amylase and cellulase.
Yeasts have wide applicability in the industrial field, as in the production of enzymes used in biocatalysts. Biocatalysts are more efficient when compared to chemical catalysts, with emphasis on hydrolytic enzymes, such as amylase, cellulase and protease. Here we focused on prospecting yeasts, with a high capacity to synthesize hydrolytic enzymes, from a continental lotic ecosystem environment in Brazil. 75 yeasts were grown in Yeast Extract-Peptone-Dextrose (YPD) medium supplemented with antibacterial and their capacity for enzymatic production was tested in specific media. Accordingly, 64 yeasts showed enzyme production capacity. From those, six showed good enzyme indexes, 3 for amylase, 2 for cellulase and 1 for protease. All showed at least one hydrolytic enzyme activity for the tested enzymes (amylase, cellulase and protease), which suggested that the yeasts are metabolically active. By sequencing the 26S gene, we identified Naganishia diffluens and Apiotrichum mycotoxinivorans as the species with highest enzyme production activities. Those species showed potential for application as biological catalysts in the biotechnological scope, collaborating in a sustainable way for the development of industrial products.