In this paper we present a list of the fish fauna of Sorocaba river sub-basin. Fish were caught with gillnets, sieves and puçás, a sort of funnel net. We listed 71 species, distributed in 7 orders, 22 families and 50 genera. Characiformes are represented by 39 species, Siluriformes 21, Perciformes 4, Gymnotiformes 3, Cyprinodontiformes 2, and Synbranchiformes and Cypriniformes by a single species each. Five species are alien. Nine species remain as an indefinite taxonomical status.