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      First detection of Cytauxzoon spp. infection in European wildcats (Felis silvestris silvestris) of Italy.

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          Abstract

          Cytauxzoonosis is an emerging, tick-transmitted, protozoan disease affecting domestic and wild felids and caused by Cytauxzoon felis, Cytauxzoon manul and Cytauxzoon spp. This study aimed to determine the presence of infection with Cytauxzoon spp. in Felis silvestris silvestris in Italy, in order to enhance the comprehension of its pattern distribution among domestic cat populations. In addition, wildcats were tested for other endemic vector-borne pathogens in Italy. The carcasses of 21 F. s. silvestris were collected from central and northern regions of Italy. All the animals were submitted to necropsy and samples of the spleens were collected. Cytauxzoon infection was surveyed by a conventional PCR amplifying a portion of the SSU-rDNA of species of Piroplasmida. The samples were also screened for Anaplasma spp., Ehrlichia spp., Rickettsia spp., Babesia spp., Theileria spp., and Leishmania spp. using SYBR Green Real-Time PCR (rPCR) assays. Four animals (19%) were positive for Piroplasmida-PCR assay and three sequenced amplicons were obtained (14.3%), clustering with the Italian, Spanish, French and Romanian Cytauxzoon spp. isolates and with C. manul found in Mongolia. The samples were negative for the other pathogens screened. The present results showed that Cytauxzoon spp. may infect both F. s. silvestris and F. s. catus.

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          Author and article information

          Journal
          Ticks Tick Borne Dis
          Ticks and tick-borne diseases
          1877-9603
          1877-959X
          Jul 2016
          : 7
          : 5
          Affiliations
          [1 ] Department of Veterinary Medicine, University of Perugia, San Costanzo Street 4, Perugia 06126, Italy. Electronic address: fabrizia.veronesi@unipg.it.
          [2 ] Istituto Zooprofilattico Sperimentale delle Venezie, University Avenue 10, Legnaro 35020, Padua, Italy.
          [3 ] School of Biosciences and Veterinary Medicine, University of Camerino, Cavour Square 19/f, Camerino 62032, Macerata, Italy.
          [4 ] Department of Veterinary Medicine, University of Perugia, San Costanzo Street 4, Perugia 06126, Italy.
          [5 ] Therion Research Group, Bracco Street 10, Trieste 34139, Italy.
          [6 ] Department of Chemistry, Biology and Biotechnologies, University of Perugia, Elce di Sotto Street 8, Perugia 06123, Italy.
          [7 ] Department of Agricultural, Food, Environmental and Animal Sciences, University of Udine, Sondrio Street 2, 33100 Udine, Italy.
          Article
          S1877-959X(16)30059-0
          10.1016/j.ttbdis.2016.04.003
          27150590
          87263477-a371-47c6-8a07-d6957efa9c1a
          Copyright © 2016 Elsevier GmbH. All rights reserved.
          History

          Cytauxzoon spp,Felis silvestris silvestris,Italy,Vector-borne pathogens

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