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      Protection of Differentiating Neuronal Cells from Amyloid β Peptide-induced Injury by Alkaline Extract of Leaves of Sasa senanensis Rehder

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          Abstract

          Background/Aim: We have previously reported the protection of doxorubicin-induced keratinocyte toxicity by alkaline extract of the leaves of Sasa senanensis Rehder (SE). In order to extend the generality of the cell protective effect of SE, we investigated whether it also protects rat PC12 and human SH-SY5Y neuron model cells from amyloid β-peptide (Aβ)-induced injury. Materials and Methods: Viability of cells was determined by the MTT method. Cytotoxicity was evaluated by the concentration that reduces the cell viability by 50% (CC 50). Protection from Aβ-induced cytotoxicity was evaluated by the concentration that reversed the Aβ-induced reduction of viability by 50% (EC 50). The selectivity index (SI) of neuroprotective activity was defined as the ratio of EC 50 to CC 50.Aβ 1-42 aggregation was assayed using Aβ 1-42> ammonium hydroxide. Results: SE showed hormetic growth stimulation at lower concentrations in both neuron precursors and differentiated cells. SE reproducibly inhibited Aβ-induced cytotoxicity against both undifferentiated and differentiated neuron cells. Both the extent of differentiation induction and viability depended on the cell density, suggesting the release of growth and differentiation stimulation substances into culture supernatant. Higher concentrations of SE partially reduced the Aβ 1-42 aggregation. Conclusion: Hormetic growth stimulation and inhibition of aggregation may be involved in the neuroprotective activity of SE.

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          Author and article information

          Journal
          In Vivo
          In Vivo
          In Vivo
          International Institute of Anticancer Research
          0258-851X
          1791-7549
          3 March 2018
          Mar-Apr 2018
          : 32
          : 2
          : 231-239
          Affiliations
          [1 ]Meikai University School of Dentistry, Saitama, Japan
          [2 ]School of Medicine, Showa University, Tokyo, Japan
          [3 ]Faculty of Pharmaceutical Sciences, Teikyo Heisei University, Tokyo, Japan
          [4 ]Daiwa Biological Research Institute Co., Ltd., Kanagawa, Japan
          Author notes
          Hiroshi Sakagami, Meikai University Research Institute of Odontology (M-RIO), Sakado, Saitama 350-0283, Japan. Tel: +81 492792758, Fax: +81 492855171 sakagami@ 123456dent.meikai.ac.jp
          Article
          PMC5905189 PMC5905189 5905189
          10.21873/invivo.11229
          5905189
          29475904
          876ab5c0-e641-4aff-8a72-13edda32fcf9
          Copyright 2018, International Institute of Anticancer Research
          History
          : 29 November 2017
          : 18 December 2017
          Categories
          Research Article

          protection,bamboo leaf extract,hormesis,amyloid β-peptide,differentiation,NGF,Nerve cell

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