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      CD9 Expression by Human Granulosa Cells and Platelets as a Predictor of Fertilization Success during IVF

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          Abstract

          Objective. To determine whether CD9 expression on human granulosa cells (GCs) and platelets could predict the success of conventional fertilization of human oocytes during in vitro fertilization (IVF). Methods. Thirty women undergoing IVF for nonmale factor infertility participated. Platelets from venous blood and GCs separated from retrieved oocytes were prepared for immunofluorescence. Flow cytometry quantified the percent of GCs expressing CD9, and CD9 surface density on GCs and platelets. Fertilization rate was determined for the total number of oocytes, and the number of mature oocytes per patient. Correlations tested for significant relationships ( P < .05) between fertilization rates and CD9 expression. Results. CD9 surface density on human GCs is inversely correlated with fertilization rate of oocytes ( P = .04), but the relationship was weak. Conclusion. More studies are needed to determine if CD9 expression on GCs would be useful for predicting conventional fertilization success during IVF.

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          Pregnancies after intracytoplasmic injection of single spermatozoon into an oocyte

          G Palermo (1992)
          Article 0 comments Cited 161 times – based on 0 reviews     Review now
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            Requirement of CD9 on the egg plasma membrane for fertilization.

            H Hasuwa, Carolyn K. Suzuki, Elizabeth S. Yamada … (2000)
            CD9 is an integral membrane protein associated with integrins and other membrane proteins. Mice lacking CD9 were produced by homologous recombination. Both male and female CD9-/- mice were born healthy and grew normally. However, the litter size from CD9-/- females was less than 2% of that of the wild type. In vitro fertilization experiments indicated that the cause of this infertility was due to the failure of sperm-egg fusion. When sperm were injected into oocytes with assisted microfertilization techniques, however, the fertilized eggs developed to term. These results indicate that CD9 has a crucial role in sperm-egg fusion.
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              Human cumulus granulosa cell gene expression: a predictor of fertilization and embryo selection in women undergoing IVF.

              Martin Matzuk, P Cisneros-Moysen, E Kovanci … (2004)
              A biochemical marker for embryo development would increase the chance of a successful pregnancy with IVF by optimizing oocyte and embryo selection, and allow fewer embryos to be transferred. In this study, we correlated cumulus granulosa cell gene expression of hyaluronic acid synthase 2 (HAS2), cyclooxygenase 2 (COX2; PTGS2) and gremlin (GREM1) with subsequent embryo development in search of a parameter for embryo selection. Cumulus cell gene expression was determined prospectively on eight consecutive patients undergoing IVF with ICSI. Immediately following oocyte retrieval, the cumulus was stripped from the oocyte, and cumulus gene expression for PTGS2, HAS2 and GREM1 was assessed using a one-step real-time quantitative RT-PCR assay. Oocyte quality, fertilization and embryo morphology were correlated to relative gene expression. Gene expression data were available on cumulus cells from 108 oocytes that developed into 70 embryos (64.8% fertilization rate). Cumulus PTGS2, HAS2 and GREM1 expression was higher from oocytes that developed into higher quality embryos (grades 3, 4 and 5) compared with lower quality embryos (grades 1 and 2) (P<0.05, P<0.001 and P<0.001, respectively). HAS2 and GREM1 expression was also higher from the cumulus surrounding oocytes that gave rise to higher grade embryos (P<0.001). The expression of PTGS2 and HAS2 was 6-fold higher, and that of GREM1 was 15-fold higher in cumulus yielding higher grade embryos versus lower grade embryos. PTGS2, HAS2 and GREM1 gene expression correlates to morphological and physiological characteristics and provides a novel approach to predict human embryo development. Ultimately, with better predictors of follicular and embryonic health, higher quality embryos can be selected and transferred, reducing higher order pregnancy rates.
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                Author and article information

                Journal
                Journal ID (nlm-ta): Obstet Gynecol Int
                Journal ID (publisher-id): OGI
                Title: Obstetrics and Gynecology International
                Publisher: Hindawi Publishing Corporation
                ISSN (Print): 1687-9589
                ISSN (Electronic): 1687-9597
                Publication date (Print): 2010
                Publication date (Electronic): 1 September 2010
                Volume: 2010
                Electronic Location Identifier: 192461
                Affiliations
                1Department of Biology, Rhodes College, 2000 North Parkway, Memphis, TN 38112, USA
                2Department of Obstetrics and Gynecology, University of Tennessee Health Science Center, Memphis, TN 38152, USA
                3Department of Internal Medicine, The Vascular Biology Center of Excellence, University of Tennessee Health Science Center, Memphis, TN 38152, USA
                4Fertility Associates of Memphis, 80 Humphreys Center, Suite 307, Memphis, TN 38120, USA
                Author notes
                *William H. Kutteh: wkutteh@ 123456uthsc.edu

                Academic Editor: Edward V. Younglai

                Article
                DOI: 10.1155/2010/192461
                PMC ID: 2938453
                PubMed ID: 20862371
                SO-VID: 879d8546-fe76-4ed5-9c25-271e92855158
                Copyright © Copyright © 2010 Carolyn R. Jaslow et al.
                License:

                This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

                History
                Date received : 27 May 2010
                Date revision received : 16 July 2010
                Date accepted : 28 July 2010
                Categories
                Subject: Research Article

                ScienceOpen disciplines: Obstetrics & Gynecology
                Data availability:
                ScienceOpen disciplines: Obstetrics & Gynecology

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