Blog
About

29
views
0
recommends
+1 Recommend
1 collections
    3
    shares
      • Record: found
      • Abstract: found
      • Article: found

      Identification, expression analysis, and functional characterization of ghrelin and its receptors in spotted sea bass ( Lateolabrax maculatus)

      Read this article at

      ScienceOpenPublisher
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Ghrelin (Ghrl), an appetite-inducing peptide hormone secreted by the stomach, is the endogenous ligand for the growth hormone secretagogue receptor (Ghs-r). In this study, we identified the preproghrelin gene and its receptors in spotted sea bass ( Lateolabrax maculatus). The ghrl gene consisted of an open reading frame (ORF) of 324 nucleotides encoding 107 amino acids, and the premature protein contained a 20-amino-acid mature peptide. Through a syntenic analysis, we also validated the annotation of growth hormone secretagogue receptor 1a (ghs-r1a) and growth hormone secretagogue receptor 1a-like (ghs-r1a-like), which contained seven-transmembrane structures, in spotted sea bass. The ORF of ghs-r1a consisted of 1152 bp that encoded a 383-amino-acid protein, and ghs-r1a-like contained an ORF of 2631 bp and produced a protein with 876 amino acids. A phylogenetic analysis showed that spotted sea bass ghrl and its receptors clustered with those of other fishes and were more distantly related to those of other vertebrates. In situ hybridization revealed that ghrl was highly expressed in the stomach and localized in the mucosa and submucosa. The expression of these genes varied during short-term starvation in a time-dependent manner. In vitro studies showed that after incubation with Ghrl for 3 h enhanced the expression of motilin ( mln), gastrin ( gas) and cholecystokinin ( cck), but this effect vanished after 6 h of incubation. In summary, Ghrl and its receptors might play important roles in the regulation of food intake in spotted sea bass.

          Related collections

          Author and article information

          Journal
          MLST
          Marine Life Science & Technology
          Springer (China )
          2096-6490
          2662-1746
          01 November 2020
          18 August 2020
          : 2
          : 4
          : 349-359
          Affiliations
          1Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, China
          Author notes
          *Corresponding authors: Yun Li, E-mail: yunli0116@ 123456ouc.edu.cn ; Haishen Wen, wenhaishen@ 123456ouc.edu.cn
          Article
          s42995-020-00055-x
          10.1007/s42995-020-00055-x
          © 2020 Ocean University of China
          Product
          Self URI (journal-page): https://www.springer.com/journal/42995
          Categories
          Research Papers

          Comments

          Comment on this article