Biology of two European Tenthredo species (Hymenoptera, Tenthredinidae) feeding on Gentiana

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Abstract

Very few sawflies using Gentianaceae as larval host plants have been recorded. We identified larvae collected in Austria on Gentiana asclepiadea L. as Tenthredo atra Linnaeus, 1758 and T. propinqua Klug, 1817. If its current taxonomic circumscription as a single species is accepted, T. atra is a highly polyphagous species, whereas T. propinqua may be more specialised: Gentiana asclepiadea is its first recorded host. We sequenced plant DNA from the head of one T. propinqua larva, which confirmed that it had been feeding on this plant. This is the first recorded use of G. asclepiadea by sawfly larvae. Larvae are illustrated, and identification characters are described.

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Universal primers for amplification of three non-coding regions of chloroplast DNA.

Pierre Taberlet, Ludovic Gielly, Guy Pautou … (1991)

Six primers for the amplification of three non-coding regions of chloroplast DNA via the polymerase chain reaction (PCR) have been designed. In order to find out whether these primers were universal, we used them in an attempt to amplify DNA from various plant species. The primers worked for most species tested including algae, bryophytes, pteridophytes, gymnosperms and angiosperms. The fact that they amplify chloroplast DNA non-coding regions over a wide taxonomic range means that these primers may be used to study the population biology (in supplying markers) and evolution (inter- and probably intraspecific phylogenies) of plants.