Serum Levels of 3-Deoxyglucosone and Tissue Contents of Advanced Glycation End Products Are Increased in Streptozotocin-Induced Diabetic Rats with Nephropathy

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Abstract

To investigate a role of the Maillard reaction in the pathogenesis of diabetic nephropathy, we measured serum levels of 3-deoxyglucosone (3-DG), a potent protein cross-linking intermediate of the Maillard reaction, and tissue contents of advanced glycation end products (AGEs) in streptozotocin (STZ)-induced diabetic rats. We quantified serum 3-DG using gas chromatography/ mass spectrometry, and measured AGE contents in tissues using a competitive enzyme-linked immunosorbent assay with a monoclonal anti-AGE antibody. The STZ-induced diabetic rats showed nephropathy with proteinuria, hypoproteinemia, hyperlipidemia and reduced creatinine clearance. Serum levels of 3-DG in the STZ-induced diabetic rats (mean ± SE; 3.46 ± 0.23 μmol/l) were significantly (p < 0.01) higher than those in control rats (1.23 ± 0.13 μmol/l). AGE contents in the kidney and the lens obtained from the STZ-induced diabetic rats (398 ± 45 and 816 ± 200 arbitrary units, respectively) were also significantly (p < 0.01) higher than those in the control rats (122 ± 10 and 299 ± 50 arbitrary units, respectively). The results indicate that increased levels of serum 3-DG and renal tissue AGEs may be related to the occurrence of diabetic nephropathy.

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Journal

Journal ID (publisher-id): NEF

Journal ID (nlm-ta): Nephron

Journal ID (doi): 10.1159/issn.1660-8151

Title: Nephron

Publisher: S. Karger AG

ISSN (Print): 1660-8151

ISSN (Electronic): 2235-3186

Publication date Subscription-year: 1996

Publication date (Print): 1996

Publication date (Electronic): 24 December 2008

Volume: 74

Issue: 3

Pages: 580-585

Affiliations

^aNagoya University Branch Hospital, Higashi-ku, Nagoya, and ^bMaruko Pharmaceutical Co., Ltd, Kodama, Nishi-ku, Nagoya, Japan

Article

Publisher ID: 189456 Other ID: Nephron 1996;74:580–585

DOI: 10.1159/000189456

PubMed ID: 8938685

SO-VID: 882a377d-cb14-48ca-a566-4ea3ce29903c

License:

Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

History

Date accepted : 22 January 1996

Page count

Pages: 6

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