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      Mutations in the deubiquitinase gene USP8 cause Cushing's disease.

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          Abstract

          Cushing's disease is caused by corticotroph adenomas of the pituitary. To explore the molecular mechanisms of endocrine autonomy in these tumors, we performed exome sequencing of 10 corticotroph adenomas. We found somatic mutations in the USP8 deubiquitinase gene in 4 of 10 adenomas. The mutations clustered in the 14-3-3 protein binding motif and enhanced the proteolytic cleavage and catalytic activity of USP8. Cleavage of USP8 led to increased deubiqutination of the EGF receptor, impairing its downregulation and sustaining EGF signaling. USP8 mutants enhanced promoter activity of the gene encoding proopiomelanocortin. In summary, our data show that dominant mutations in USP8 cause Cushing's disease via activation of EGF receptor signaling.

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          Author and article information

          Journal
          Nat. Genet.
          Nature genetics
          1546-1718
          1061-4036
          Jan 2015
          : 47
          : 1
          Affiliations
          [1 ] Medizinische Klinik und Poliklinik IV, Ludwig-Maximilians-Universität München, Munich, Germany.
          [2 ] 1] Medizinische Klinik und Poliklinik IV, Ludwig-Maximilians-Universität München, Munich, Germany. [2] Department of Medicine I, Endocrine and Diabetes Unit, University Hospital, University of Würzburg, Würzburg, Germany.
          [3 ] Department of Biological Sciences, Tokyo Institute of Technology, Yokohama, Japan.
          [4 ] Department of Endocrinology, Max Planck Institute of Psychiatry, Munich, Germany.
          [5 ] 1] Institute of Human Genetics, Helmholtz Zentrum München, Neuherberg, Germany. [2] Institute of Human Genetics, Technische Universität München, Munich, Germany. [3] DZHK (German Centre for Cardiovascular Research) partner site, Munich Heart Alliance, Munich, Germany.
          [6 ] Laboratory of Protein Metabolism, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan.
          [7 ] Institute of Human Genetics, Helmholtz Zentrum München, Neuherberg, Germany.
          [8 ] Institut für Neuropathologie der Universität Hamburg, Hamburg, Germany.
          [9 ] Comprehensive Cancer Center Mainfranken, University of Würzburg, Würzburg, Germany.
          [10 ] 1] Department of Medicine I, Endocrine and Diabetes Unit, University Hospital, University of Würzburg, Würzburg, Germany. [2] Comprehensive Heart Failure Center, University of Würzburg, Würzburg, Germany.
          [11 ] Neurochirurgische Klinik, Klinikum der Universität Erlangen, Erlangen, Germany.
          [12 ] 1] Institute of Human Genetics, Helmholtz Zentrum München, Neuherberg, Germany. [2] Institute of Human Genetics, Technische Universität München, Munich, Germany.
          [13 ] 1] Medizinische Klinik und Poliklinik IV, Ludwig-Maximilians-Universität München, Munich, Germany. [2] Department of Medicine I, Endocrine and Diabetes Unit, University Hospital, University of Würzburg, Würzburg, Germany. [3] Comprehensive Cancer Center Mainfranken, University of Würzburg, Würzburg, Germany.
          Article
          ng.3166
          10.1038/ng.3166
          25485838
          883fc8d0-a62d-4354-81e0-762cf77949dc
          History

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