Purification and Characterization of a Novel Lipase from Antarctic Krill

Abstract

Lipase from Antarctic krill, with a molecular weight of 71.27 kDa, was purified with ammonium sulfate precipitation and a series of chromatographic separations over ion exchange (DEAE) and gel filtration columns (Sephacryl S-100), resulting in 5.2% recovery with a 22.4-fold purification ratio. The optimal pH and temperature for enzyme activity were 8.0 and 45°C, respectively. Purified lipase had K _m and V _max values of 3.27 mmol L ⁻¹ and 2.4 U mg ⁻¹, respectively, using p-nitrophenyl laurate as the substrate. Lipase activity was enhanced by adding Ca ²⁺ and Mg ²⁺ ions in the concentration ranges of 0–0.5 mmol L ⁻¹ and 0–0.3 mmol L ⁻¹, respectively, while the activity was inhibited by a further increase in these ion concentrations. Fe ³⁺ and Cu ²⁺ ions showed obvious inhibitory effects on enzyme activity, and the inhibition rates were 71.8% and 53.3% when the ion concentrations were 0.5 mmol L ⁻¹.

Journal

Journal ID (publisher-id): JOUC

Title: Journal of Ocean University of China

Publisher: Science Press and Springer (China )

ISSN (Electronic): 1672-5182

Publication date (Electronic): 20 December 2019

Publication date (Print): 01 February 2020

Volume: 19

Issue: 1

Pages: 209-215

Affiliations

¹College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China

²Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Sustainable Development of Polar Fishery, Ministry of Agriculture and Rural Affairs of PRC, Qingdao 266071, China

³Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China

⁴Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China

⁵College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao 266100, China

Author notes

*Corresponding authors: XU Jiakun, E-mail: xujk@ 123456ysfri.ac.cn ; ZHAO Xianyong, E-mail: zhaoxy@ 123456ysfri.ac.cn

Article

Publisher ID: s11802-019-4174-1

DOI: 10.1007/s11802-019-4174-1

Copyright statement: Copyright © Ocean University of China, Science Press and Springer-Verlag GmbH Germany 2020.

License:

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Product

Self URI (journal-page): https://www.springer.com/journal/11802